RRC ID 69448
著者 Chai H, Hazawa M, Hosokawa Y, Igarashi J, Suga H, Kashiwakura I.
タイトル Novel acridine-based N-acyl-homoserine lactone analogs induce endoreduplication in the human oral squamous carcinoma cell line SAS.
ジャーナル Biol Pharm Bull
Abstract The cytotoxicity of novel acridine-based N-acyl-homoserine lactone (AHL) analogs was investigated on the human oral squamous carcinoma cell line SAS. One analog induced G2/M phase arrest at 5.3-10.6 µM and induced polyploidy at a higher dose (21.2 µM). Importantly, treatment of SAS cells with a combination of the AHL analog and the Jun N-terminal kinase (JNK) inhibitor, SP600125, prevented mitosis and induced polyploidy. The AHL analog synergized with X-irradiation to inhibit clonogenic survival of SAS cells; however, its radiosensitizing effects were relative to not X-irradiation-induced apoptosis but mitotic failure following enhanced expression of Aurora A and B. These results suggest that the active AHL analog showed growth-suppressive and radiosensitizing effects, which involve polyploidy followed by G2/M accumulation and atypical cell death in the SAS cell line.
巻・号 35(8)
ページ 1257-63
公開日 2012-1-1
DOI 10.1248/bpb.b12-00033
PII DN/JST.JSTAGE/bpb/b12-00033
PMID 22863922
MeSH Acridines / pharmacology Acridines / therapeutic use* Acyl-Butyrolactones / pharmacology Acyl-Butyrolactones / therapeutic use* Anthracenes / pharmacology Antineoplastic Agents / pharmacology Antineoplastic Agents / therapeutic use* Carcinoma, Squamous Cell / drug therapy* Carcinoma, Squamous Cell / metabolism Carcinoma, Squamous Cell / radiotherapy Cell Cycle Checkpoints / drug effects Cell Death / drug effects Cell Line, Tumor Cell Survival / drug effects Endoreduplication / drug effects* Humans JNK Mitogen-Activated Protein Kinases / antagonists & inhibitors Mitosis / drug effects Mouth Neoplasms / drug therapy* Mouth Neoplasms / metabolism Mouth Neoplasms / radiotherapy Polyploidy Protein Serine-Threonine Kinases / metabolism Radiation-Sensitizing Agents / pharmacology Radiation-Sensitizing Agents / therapeutic use*
IF 1.863
リソース情報
ヒト・動物細胞 SAS(RCB1974)