RRC ID 6947
著者 Kaplan OI, Molla-Herman A, Cevik S, Ghossoub R, Kida K, Kimura Y, Jenkins P, Martens JR, Setou M, Benmerah A, Blacque OE.
タイトル The AP-1 clathrin adaptor facilitates cilium formation and functions with RAB-8 in C. elegans ciliary membrane transport.
ジャーナル J Cell Sci
Abstract Clathrin adaptor (AP) complexes facilitate membrane trafficking between subcellular compartments. One such compartment is the cilium, whose dysfunction underlies disorders classified as ciliopathies. Although AP-1mu subunit (UNC-101) is linked to cilium formation and targeting of transmembrane proteins (ODR-10) to nematode sensory cilia at distal dendrite tips, these functions remain poorly understood. Here, using Caenorhabditis elegans sensory neurons and mammalian cell culture models, we find conservation of AP-1 function in facilitating cilium morphology, positioning and orientation, and microtubule stability and acetylation. These defects appear to be independent of IFT, because AP-1-depleted cells possess normal IFT protein localisation and motility. By contrast, disruption of chc-1 (clathrin) or rab-8 phenocopies unc-101 worms, preventing ODR-10 vesicle formation and causing misrouting of ODR-10 to all plasma membrane destinations. Finally, ODR-10 colocalises with RAB-8 in cell soma and they cotranslocate along dendrites, whereas ODR-10 and UNC-101 signals do not overlap. Together, these data implicate conserved roles for metazoan AP-1 in facilitating cilium structure and function, and suggest cooperation with RAB-8 to coordinate distinct early steps in neuronal ciliary membrane sorting and trafficking.
巻・号 123(Pt 22)
ページ 3966-77
公開日 2010-11-15
DOI 10.1242/jcs.073908
PII jcs.073908
PMID 20980383
PMC PMC2972276
MeSH Adaptor Protein Complex 1 / metabolism Adaptor Protein Complex 1 / physiology* Animals Biological Transport Caenorhabditis elegans / metabolism Caenorhabditis elegans / physiology* Cell Membrane / metabolism Cilia / metabolism Cilia / ultrastructure Clathrin / metabolism
IF 4.573
引用数 39
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm2526 tm3288 tm324