論文 - 詳細
| RRC ID | 69507 |
|---|---|
| 著者 | Chacha PP, Horie R, Kusakabe TG, Sasakura Y, Singh M, Horie T, Levine M. |
| タイトル | Neuronal identities derived by misexpression of the POU IV sensory determinant in a protovertebrate. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
The protovertebrate Ciona intestinalis type A (sometimes called Ciona robusta) contains a series of sensory cell types distributed across the head-tail axis of swimming tadpoles. They arise from lateral regions of the neural plate that exhibit properties of vertebrate placodes and neural crest. The sensory determinant POU IV/Brn3 is known to work in concert with regional determinants, such as Foxg and Neurogenin, to produce palp sensory cells (PSCs) and bipolar tail neurons (BTNs), in head and tail regions, respectively. A combination of single-cell RNA-sequencing (scRNA-seq) assays, computational analysis, and experimental manipulations suggests that misexpression of POU IV results in variable transformations of epidermal cells into hybrid sensory cell types, including those exhibiting properties of both PSCs and BTNs. Hybrid properties are due to coexpression of Foxg and Neurogenin that is triggered by an unexpected POU IV feedback loop. Hybrid cells were also found to express a synthetic gene battery that is not coexpressed in any known cell type. We discuss these results with respect to the opportunities and challenges of reprogramming cell types through the targeted misexpression of cellular determinants. |
| 巻・号 | 119(4) |
| 公開日 | 2022-1-25 |
| DOI | 10.1073/pnas.2118817119 |
| PII | 2118817119 |
| PMID | 35042818 |
| PMC | PMC8794889 |
| MeSH | Animals Biological Evolution Cellular Reprogramming / genetics Cellular Reprogramming / physiology Ciona intestinalis / genetics* Ciona intestinalis / metabolism Epidermis / innervation Epidermis / metabolism Gene Expression / genetics Gene Expression Regulation, Developmental / genetics Gene Regulatory Networks / genetics Neural Crest / metabolism Neural Plate / metabolism Neurons / metabolism* POU Domain Factors / genetics POU Domain Factors / metabolism* Single-Cell Analysis Transcription Factors / metabolism Vertebrates / genetics |
| IF | 9.412 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カタユウレイボヤ・(ニッポンウミシダ) | Wild C. int Tg[MiCiPC2K]3 Tg[MiCiPC2K]2 |