Reference - Detail
| RRC ID | 69571 |
|---|---|
| Author | Ota Y, Takahashi K, Otake S, Tamaki Y, Okada M, Yan I, Aso K, Fujii S, Patel T, Haneda M. |
| Title | Extracellular RNA transfer from non-malignant human cholangiocytes can promote cholangiocarcinoma growth. |
| Journal | FEBS Open Bio |
| Abstract |
Extracellular vesicles (EV) within the cellular secretome are emerging as modulators of pathological processes involved in tumor growth through their ability to transfer donor-derived RNA into recipient cells. While the effects of tumor and stromal cell EVs within the tumor microenvironment have been studied, less is known about the contributions of normal, nontransformed cells. We examined the impact of EVs within the cellular secretome from nonmalignant cells on transformed cell growth and behavior in cholangiocarcinoma cells. These effects were enhanced in the presence of the pro-fibrogenic mediator TGF-β. We identified miR-195 as a TGF-β responsive miRNA in normal cells that can be transferred via EV to tumor cells and regulate cell growth, invasion, and migration. The effects of miR-195 involve modulation of the epithelial-mesenchymal transition through direct effects on the transcription factor Snail. These studies provide in vitro and in vivo evidence for the impact of normal cellular secretome on transformed cell growth, show the importance of EV RNA transfer, and identify mechanisms of EV-mediated transfer of miRNA as a contributor to tumor development, which may provide new therapeutic opportunities for targeting human cholangiocarcinoma. |
| Volume | 11(12) |
| Pages | 3276-3292 |
| Published | 2021-12-1 |
| DOI | 10.1002/2211-5463.13294 |
| PMID | 34510808 |
| PMC | PMC8634862 |
| MeSH | Cell Line, Tumor Cell Movement / genetics Cell Proliferation / genetics Cholangiocarcinoma / genetics* Cholangiocarcinoma / metabolism Cholangiocarcinoma / physiopathology Epithelial Cells / metabolism Epithelial-Mesenchymal Transition / genetics Extracellular Vesicles / genetics* Extracellular Vesicles / physiology Gene Expression Regulation / genetics Humans MicroRNAs / genetics* MicroRNAs / metabolism Neoplasm Invasiveness / genetics Secretome Signal Transduction / genetics Tumor Microenvironment / genetics |
| IF | 2.231 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | RBE(RCB1292) TFK-1(RCB2537) |