RRC ID 69599
Author Kageyama T, Nagashio R, Ryuge S, Matsumoto T, Iyoda A, Satoh Y, Masuda N, Jiang SX, Saegusa M, Sato Y.
Title HADHA is a potential predictor of response to platinum-based chemotherapy for lung cancer.
Journal Asian Pac J Cancer Prev
Abstract To identify a cisplatin resistance predictor to reduce or prevent unnecessary side effects, we firstly established four cisplatin-resistant sub-lines and compared their protein profiles with cisplatin-sensitive parent lung cancer cell lines using two-dimensional gel electrophoresis. Between the cisplatin-resistant and -sensitive cells, a total of 359 protein spots were differently expressed (>1.5 fold), and 217 proteins (83.0%) were identified. We focused on a mitochondrial protein, hydroxyl-coenzyme A dehydrogenase/3-ketoacyl-coenzyme A thiolase/enoyl-coenzyme A hydratase alpha subunit (HADHA), which was increased in all cisplatin-resistant cells. Furthermore, pre- treated biopsy specimens taken from patients who showed resistance to platinum-based treatment showed a significantly higher positive rate for HADHA in all cases (p=0.00367), including non-small cell lung carcinomas (p=0.002), small-cell lung carcinomas (p=0.038), and adenocarcinomas (p=0.008). These results suggest that the expression of HADHA may be a useful marker to predict resistance to platinum-based chemotherapy in patients with lung cancer.
Volume 12(12)
Pages 3457-63
Published 2011-1-1
PMID 22471497
MeSH Adenocarcinoma / drug therapy* Adenocarcinoma / enzymology Adenocarcinoma / pathology Antineoplastic Combined Chemotherapy Protocols / therapeutic use* Blotting, Western Camptothecin / administration & dosage Camptothecin / analogs & derivatives Carboplatin / administration & dosage Carcinoma, Non-Small-Cell Lung / drug therapy* Carcinoma, Non-Small-Cell Lung / enzymology Carcinoma, Non-Small-Cell Lung / pathology Carcinoma, Squamous Cell / drug therapy* Carcinoma, Squamous Cell / enzymology Carcinoma, Squamous Cell / pathology Cisplatin / administration & dosage Deoxycytidine / administration & dosage Deoxycytidine / analogs & derivatives Electrophoresis, Gel, Two-Dimensional Etoposide / administration & dosage Follow-Up Studies Gemcitabine Humans Irinotecan Lung Neoplasms / drug therapy* Lung Neoplasms / enzymology Lung Neoplasms / pathology Mitochondrial Trifunctional Protein Mitochondrial Trifunctional Protein, alpha Subunit Multienzyme Complexes / metabolism* Neoplasm Recurrence, Local / drug therapy Neoplasm Recurrence, Local / enzymology Neoplasm Recurrence, Local / pathology Paclitaxel / administration & dosage Prognosis Small Cell Lung Carcinoma / drug therapy* Small Cell Lung Carcinoma / enzymology Small Cell Lung Carcinoma / pathology Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tumor Cells, Cultured
Human and Animal Cells LC-2/ad(RCB0440)