RRC ID |
69607
|
Author |
Tien PG, Kayama F, Konishi F, Tamemoto H, Kasono K, Hung NT, Kuroki M, Ishikawa SE, Van CN, Kawakami M.
|
Title |
Inhibition of tumor growth and angiogenesis by water extract of Gac fruit (Momordica cochinchinensis Spreng).
|
Journal |
Int J Oncol
|
Abstract |
The antitumor activity of the crude water extract from Gac fruit (Momordica cochinchinensis) was investigated in vivo and in vitro. A water extract prepared from 0.75 and 0.25 mg dry weight of Gac fruit per gram body weight was given daily to Balb/c mice (n=15/group). The water extract inhibited the growth of the colon 26-20 adenocarcinoma cell line, transplanted in Balb/c mice, reducing wet tumor weight by 23.6%. Histological and immunohistochemical results indicated that Gac water extract reduced the density of blood vessels around the carcinoma. The water extract also produced a marked suppression of cell proliferation in colon 26-20 and HepG2 cells. Cell cycle analysis demonstrated a significant accumulation of cells in the S phase by water extract. Immunoblotting showed that cyclin A, Cdk2, p27waf1/Kip1 were down-regulated, whereas the protein level of p21waf1/Cip1 was not decreased. Treatment of colon 26-20 cells with Gac extract induced necrosis rather than apoptosis. The antitumor component was confirmed as a protein with molecular weight of 35 kDa, retained in the water-soluble high molecular weight fraction. Thus, the bioactive antitumor compound in Gac extract is a protein, which is distinct from lycopene, another compound in Gac fruit with potential antitumor activity.
|
Volume |
26(4)
|
Pages |
881-9
|
Published |
2005-4-1
|
PMID |
15753981
|
MeSH |
Adenocarcinoma / pathology*
Animals
Carcinoma, Hepatocellular / pathology*
Cell Cycle / drug effects*
Cell Cycle Proteins / biosynthesis
Cell Proliferation / drug effects*
Colonic Neoplasms / pathology*
Cyclin A / biosynthesis
Cyclin-Dependent Kinase Inhibitor p21
Down-Regulation
Drug Screening Assays, Antitumor
Immunoblotting
Liver Neoplasms / pathology*
Male
Mice
Mice, Inbred BALB C
Momordica / chemistry*
Necrosis
Neovascularization, Pathologic*
Plant Extracts / pharmacology*
Transplantation, Heterologous
Tumor Cells, Cultured
Water / chemistry
|
IF |
3.899
|
Resource |
Human and Animal Cells |
Hep G2 |