論文 - 詳細
| RRC ID | 69607 |
|---|---|
| 著者 | Tien PG, Kayama F, Konishi F, Tamemoto H, Kasono K, Hung NT, Kuroki M, Ishikawa SE, Van CN, Kawakami M. |
| タイトル | Inhibition of tumor growth and angiogenesis by water extract of Gac fruit (Momordica cochinchinensis Spreng). |
| ジャーナル | Int J Oncol |
| Abstract |
The antitumor activity of the crude water extract from Gac fruit (Momordica cochinchinensis) was investigated in vivo and in vitro. A water extract prepared from 0.75 and 0.25 mg dry weight of Gac fruit per gram body weight was given daily to Balb/c mice (n=15/group). The water extract inhibited the growth of the colon 26-20 adenocarcinoma cell line, transplanted in Balb/c mice, reducing wet tumor weight by 23.6%. Histological and immunohistochemical results indicated that Gac water extract reduced the density of blood vessels around the carcinoma. The water extract also produced a marked suppression of cell proliferation in colon 26-20 and HepG2 cells. Cell cycle analysis demonstrated a significant accumulation of cells in the S phase by water extract. Immunoblotting showed that cyclin A, Cdk2, p27waf1/Kip1 were down-regulated, whereas the protein level of p21waf1/Cip1 was not decreased. Treatment of colon 26-20 cells with Gac extract induced necrosis rather than apoptosis. The antitumor component was confirmed as a protein with molecular weight of 35 kDa, retained in the water-soluble high molecular weight fraction. Thus, the bioactive antitumor compound in Gac extract is a protein, which is distinct from lycopene, another compound in Gac fruit with potential antitumor activity. |
| 巻・号 | 26(4) |
| ページ | 881-9 |
| 公開日 | 2005-4-1 |
| PMID | 15753981 |
| MeSH | Adenocarcinoma / pathology* Animals Carcinoma, Hepatocellular / pathology* Cell Cycle / drug effects* Cell Cycle Proteins / biosynthesis Cell Proliferation / drug effects* Colonic Neoplasms / pathology* Cyclin A / biosynthesis Cyclin-Dependent Kinase Inhibitor p21 Down-Regulation Drug Screening Assays, Antitumor Immunoblotting Liver Neoplasms / pathology* Male Mice Mice, Inbred BALB C Momordica / chemistry* Necrosis Neovascularization, Pathologic* Plant Extracts / pharmacology* Transplantation, Heterologous Tumor Cells, Cultured Water / chemistry |
| IF | 3.899 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2 |