RRC ID 69607
Author Tien PG, Kayama F, Konishi F, Tamemoto H, Kasono K, Hung NT, Kuroki M, Ishikawa SE, Van CN, Kawakami M.
Title Inhibition of tumor growth and angiogenesis by water extract of Gac fruit (Momordica cochinchinensis Spreng).
Journal Int J Oncol
Abstract The antitumor activity of the crude water extract from Gac fruit (Momordica cochinchinensis) was investigated in vivo and in vitro. A water extract prepared from 0.75 and 0.25 mg dry weight of Gac fruit per gram body weight was given daily to Balb/c mice (n=15/group). The water extract inhibited the growth of the colon 26-20 adenocarcinoma cell line, transplanted in Balb/c mice, reducing wet tumor weight by 23.6%. Histological and immunohistochemical results indicated that Gac water extract reduced the density of blood vessels around the carcinoma. The water extract also produced a marked suppression of cell proliferation in colon 26-20 and HepG2 cells. Cell cycle analysis demonstrated a significant accumulation of cells in the S phase by water extract. Immunoblotting showed that cyclin A, Cdk2, p27waf1/Kip1 were down-regulated, whereas the protein level of p21waf1/Cip1 was not decreased. Treatment of colon 26-20 cells with Gac extract induced necrosis rather than apoptosis. The antitumor component was confirmed as a protein with molecular weight of 35 kDa, retained in the water-soluble high molecular weight fraction. Thus, the bioactive antitumor compound in Gac extract is a protein, which is distinct from lycopene, another compound in Gac fruit with potential antitumor activity.
Volume 26(4)
Pages 881-9
Published 2005-4-1
PMID 15753981
MeSH Adenocarcinoma / pathology* Animals Carcinoma, Hepatocellular / pathology* Cell Cycle / drug effects* Cell Cycle Proteins / biosynthesis Cell Proliferation / drug effects* Colonic Neoplasms / pathology* Cyclin A / biosynthesis Cyclin-Dependent Kinase Inhibitor p21 Down-Regulation Drug Screening Assays, Antitumor Immunoblotting Liver Neoplasms / pathology* Male Mice Mice, Inbred BALB C Momordica / chemistry* Necrosis Neovascularization, Pathologic* Plant Extracts / pharmacology* Transplantation, Heterologous Tumor Cells, Cultured Water / chemistry
IF 3.899
Human and Animal Cells Hep G2