RRC ID 69682
Author Sun-Wada GH, Tabata H, Wada Y.
Title Vacuolar-type proton ATPase is required for maintenance of apicobasal polarity of embryonic visceral endoderm.
Journal Sci Rep
Abstract The endocytic compartments keep their interior acidic through the inward flow of protons and anions from the cytosol. Acidification is mediated by a proton pump known as vacuolar-type ATPase (V-ATPase) and transporters conferring anion conductance to the organellar membrane. In this study, we analysed the phenotype of mouse embryos lacking the V-ATPase c-subunit. The mutant embryos differentiated embryonic epithelial tissues, primitive endoderm, epiblast, and extraembryonic ectoderm; however, the organisation of these epithelia was severely affected. The apical-basal polarity in the visceral endoderm layer was not properly established in the mutant embryos, resulting in abnormal epithelial morphology. Thus, the function of V-ATPase is imperative for the establishment and/or maintenance of epithelial cell polarity, which is required for early embryogenesis.
Volume 11(1)
Pages 19355
Published 2021-9-29
DOI 10.1038/s41598-021-98952-3
PII 10.1038/s41598-021-98952-3
PMID 34588579
PMC PMC8481250
MeSH Animals Cell Differentiation Cell Polarity Embryo, Mammalian Endoderm / embryology* Female Mice Mice, Knockout Mutation Pregnancy Vacuolar Proton-Translocating ATPases / genetics Vacuolar Proton-Translocating ATPases / metabolism*
IF 3.998
Mice RBRC03983