RRC ID 69741
Author Nii T, Konno K, Matsumoto M, Bhukhai K, Borwornpinyo S, Sakai K, Hongeng S, Sugiyama D.
Title The Bioactive Peptide SL-13R Expands Human Umbilical Cord Blood Hematopoietic Stem and Progenitor Cells In Vitro.
Journal Molecules
Abstract Hematopoietic stem and progenitor cell (HSPC) transplantation is a curative treatment of hematological disorders that has been utilized for several decades. Although umbilical cord blood (UCB) is a promising source of HSPCs, the low dose of HSPCs in these preparations limits their use, prompting need for ex vivo HSPC expansion. To establish a more efficient method to expand UCB HSPCs, we developed the bioactive peptide named SL-13R and cultured UCB HSPCs (CD34+ cells) with SL-13R in animal component-free medium containing a cytokine cocktail. Following 9 days of culture with SL-13R, the numbers of total cells, CD34+, CD38- cells, and hematopoietic stem cell (HSC)-enriched cells were significantly increased relative to control. Transplantation of cells cultured with SL-13R into immunodeficient NOD/Shi-scid/IL-2Rγ knockout mice confirmed that they possess long-term reconstitution and self-renewal ability. AHNAK, ANXA2, and PLEC all interact with SL-13R. Knockdown of these genes in UCB CD34+ cells resulted in reduced numbers of hematopoietic colonies relative to SL-13R-treated and non-knockdown controls. In summary, we have identified a novel bioactive peptide SL-13R promoting expansion of UCB CD34+ cells with long-term reconstitution and self-renewal ability, suggesting its clinical use in the future.
Volume 26(7)
Published 2021-4-1
DOI 10.3390/molecules26071995
PII molecules26071995
PMID 33915948
PMC PMC8036704
MeSH Animals Antigens, CD34 / metabolism Biomarkers Carrier Proteins Cell Culture Techniques Cell Differentiation Cell Self Renewal Cells, Cultured Fetal Blood / cytology* Fluorescent Antibody Technique Hematopoietic Stem Cell Transplantation Hematopoietic Stem Cells / cytology* Hematopoietic Stem Cells / drug effects* Hematopoietic Stem Cells / metabolism Humans Immunophenotyping Mice Peptides / pharmacology* Protein Binding
IF 3.267
Cord blood stem cells for research