論文 - 詳細
| RRC ID | 69763 |
|---|---|
| 著者 | Azuma YT, Fujita T, Izawa T, Hirota K, Nishiyama K, Ikegami A, Aoyama T, Ike M, Ushikai Y, Kuwamura M, Fujii H, Tsuneyama K. |
| タイトル | IL-19 Contributes to the Development of Nonalcoholic Steatohepatitis by Altering Lipid Metabolism. |
| ジャーナル | Cells |
| Abstract |
Interleukin (IL)-19, a member of the IL-10 family, is an anti-inflammatory cytokine produced primarily by macrophages. Nonalcoholic steatohepatitis (NASH) is a disease that has progressed from nonalcoholic fatty liver disease (NAFLD) and is characterized by inflammation and fibrosis. We evaluated the functions of IL-19 in a NAFLD/NASH mouse model using a 60% high fat diet with 0.1% methionine, without choline, and with 2% cholesterol (CDAHFD). Wild-type (WT) and IL-19 gene-deficient (KO) mice were fed a CDAHFD or standard diet for 9 weeks. Liver injury, inflammation, and fibrosis induced by CDAHFD were significantly worse in IL-19 KO mice than in WT mice. IL-6, TNF-α, and TGF-β were significantly higher in IL-19 KO mice than in WT mice. As a mechanism using an in vitro experiment, palmitate-induced triglyceride and cholesterol contents were decreased by the addition of IL-19 in HepG2 cells. Furthermore, addition of IL-19 decreased the expression of fatty acid synthesis-related enzymes and increased ATP content in HepG2 cells. The action of IL-19 in vitro suppressed lipid metabolism. In conclusion, IL-19 may play an important role in the development of steatosis and fibrosis by directly regulating liver metabolism and may be a potential target for the treatment of liver diseases. |
| 巻・号 | 10(12) |
| 公開日 | 2021-12-13 |
| DOI | 10.3390/cells10123513 |
| PII | cells10123513 |
| PMID | 34944021 |
| PMC | PMC8699936 |
| MeSH | Adenosine Triphosphate / metabolism Animals Cholesterol / metabolism Diet, High-Fat / adverse effects Disease Models, Animal Fatty Acids / biosynthesis Fatty Acids / genetics Hep G2 Cells Humans Inflammation / etiology Inflammation / genetics* Inflammation / metabolism Inflammation / pathology Interleukin-6 / genetics Interleukins / genetics* Lipid Metabolism / genetics* Liver / injuries Liver / metabolism Liver / pathology Mice Mice, Knockout Non-alcoholic Fatty Liver Disease / etiology Non-alcoholic Fatty Liver Disease / genetics* Non-alcoholic Fatty Liver Disease / metabolism Non-alcoholic Fatty Liver Disease / pathology Palmitates / pharmacology Transforming Growth Factor beta / genetics Triglycerides / metabolism Tumor Necrosis Factor-alpha / genetics |
| IF | 4.366 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2 |