RRC ID 69790
Author Chang TK, Zhong YH, Liu SC, Huang CC, Tsai CH, Lee HP, Wang SW, Hsu CJ, Tang CH.
Title Apelin Promotes Endothelial Progenitor Cell Angiogenesis in Rheumatoid Arthritis Disease via the miR-525-5p/Angiopoietin-1 Pathway.
Journal Front Immunol
Abstract Angiogenesis is a critical process in the formation of new capillaries and a key participant in rheumatoid arthritis (RA) pathogenesis. The adipokine apelin (APLN) plays critical roles in several cellular functions, including angiogenesis. We report that APLN treatment of RA synovial fibroblasts (RASFs) increased angiopoietin-1 (Ang1) expression. Ang1 antibody abolished endothelial progenitor cell (EPC) tube formation and migration in conditioned medium from APLN-treated RASFs. We also found significantly higher levels of APLN and Ang1 expression in synovial fluid from RA patients compared with those with osteoarthritis. APLN facilitated Ang1-dependent EPC angiogenesis by inhibiting miR-525-5p synthesis via phospholipase C gamma (PLCγ) and protein kinase C alpha (PKCα) signaling. Importantly, infection with APLN shRNA mitigated EPC angiogenesis, articular swelling, and cartilage erosion in ankle joints of mice with collagen-induced arthritis. APLN is therefore a novel therapeutic target for RA.
Volume 12
Pages 737990
Published 2021-1-1
DOI 10.3389/fimmu.2021.737990
PMID 34659230
PMC PMC8511637
MeSH 3T3 Cells Angiopoietin-1 / genetics Angiopoietin-1 / metabolism* Animals Apelin / genetics Apelin / metabolism* Arthritis, Experimental / genetics Arthritis, Experimental / metabolism* Arthritis, Experimental / pathology Arthritis, Experimental / prevention & control Arthritis, Rheumatoid / genetics Arthritis, Rheumatoid / metabolism* Arthritis, Rheumatoid / pathology Chick Embryo Endothelial Progenitor Cells / metabolism* Endothelial Progenitor Cells / pathology Fibroblasts / metabolism* Fibroblasts / pathology Humans Mice MicroRNAs / genetics MicroRNAs / metabolism* Neovascularization, Pathologic* RNA Interference Signal Transduction Synoviocytes / metabolism* Synoviocytes / pathology
IF 5.085
Human and Animal Cells MH7A(RCB1512)