論文 - 詳細
| RRC ID | 69790 |
|---|---|
| 著者 | Chang TK, Zhong YH, Liu SC, Huang CC, Tsai CH, Lee HP, Wang SW, Hsu CJ, Tang CH. |
| タイトル | Apelin Promotes Endothelial Progenitor Cell Angiogenesis in Rheumatoid Arthritis Disease via the miR-525-5p/Angiopoietin-1 Pathway. |
| ジャーナル | Front Immunol |
| Abstract |
Angiogenesis is a critical process in the formation of new capillaries and a key participant in rheumatoid arthritis (RA) pathogenesis. The adipokine apelin (APLN) plays critical roles in several cellular functions, including angiogenesis. We report that APLN treatment of RA synovial fibroblasts (RASFs) increased angiopoietin-1 (Ang1) expression. Ang1 antibody abolished endothelial progenitor cell (EPC) tube formation and migration in conditioned medium from APLN-treated RASFs. We also found significantly higher levels of APLN and Ang1 expression in synovial fluid from RA patients compared with those with osteoarthritis. APLN facilitated Ang1-dependent EPC angiogenesis by inhibiting miR-525-5p synthesis via phospholipase C gamma (PLCγ) and protein kinase C alpha (PKCα) signaling. Importantly, infection with APLN shRNA mitigated EPC angiogenesis, articular swelling, and cartilage erosion in ankle joints of mice with collagen-induced arthritis. APLN is therefore a novel therapeutic target for RA. |
| 巻・号 | 12 |
| ページ | 737990 |
| 公開日 | 2021-1-1 |
| DOI | 10.3389/fimmu.2021.737990 |
| PMID | 34659230 |
| PMC | PMC8511637 |
| MeSH | 3T3 Cells Angiopoietin-1 / genetics Angiopoietin-1 / metabolism* Animals Apelin / genetics Apelin / metabolism* Arthritis, Experimental / genetics Arthritis, Experimental / metabolism* Arthritis, Experimental / pathology Arthritis, Experimental / prevention & control Arthritis, Rheumatoid / genetics Arthritis, Rheumatoid / metabolism* Arthritis, Rheumatoid / pathology Chick Embryo Endothelial Progenitor Cells / metabolism* Endothelial Progenitor Cells / pathology Fibroblasts / metabolism* Fibroblasts / pathology Humans Mice MicroRNAs / genetics MicroRNAs / metabolism* Neovascularization, Pathologic* RNA Interference Signal Transduction Synoviocytes / metabolism* Synoviocytes / pathology |
| IF | 5.085 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MH7A(RCB1512) |