Reference - Detail
| RRC ID | 69800 |
|---|---|
| Author | Rawat SS, Zimmerman C, Johnson BT, Cho E, Lockett SJ, Blumenthal R, Puri A. |
| Title | Restricted lateral mobility of plasma membrane CD4 impairs HIV-1 envelope glycoprotein mediated fusion. |
| Journal | Mol Membr Biol |
| Abstract |
We investigated the effect of receptor mobility on HIV-1 envelope glycoprotein (Env)-triggered fusion using B16 mouse melanoma cells that are engineered to express CD4 and CXCR4 or CCR5. These engineered cells are resistant to fusion mediated CD4-dependent HIV-1 envelope glycoprotein. Receptor mobility was measured by fluorescence recovery after photobleaching (FRAP) using either fluorescently-labeled antibodies or transient expression of GFP-tagged receptors in the cells. No significant differences between B16 and NIH3T3 (fusion-permissive) cells were seen in lateral mobility of CCR5 or lipid probes. By contrast CD4 mobility in B16 cells was about seven-fold reduced compared to its mobility in fusion-permissive NIH3T3 cells. However, a CD4 mutant (RA5) that localizes to non-raft membrane microdomains exhibited a three-fold increased mobility in B16 cells as compared with WT-CD4. Interestingly, the B16 cells expressing the RA5 mutant (but not the wild type CD4) and coreceptors supported HIV-1 Env-mediated fusion. Our data demonstrate that the lateral mobility of CD4 is an important determinant of HIV-1 fusion/entry. |
| Volume | 25(1) |
| Pages | 83-94 |
| Published | 2008-1-1 |
| DOI | 10.1080/09687680701613713 |
| PII | 783706423 |
| PMID | 18097956 |
| PMC | PMC3466082 |
| MeSH | Animals CD4 Antigens / genetics CD4 Antigens / metabolism* Gene Products, env / genetics Gene Products, env / metabolism* HIV-1 / physiology* HeLa Cells Humans Membrane Fluidity / physiology* Mice Mutation NIH 3T3 Cells Receptors, CCR5 / genetics Receptors, CCR5 / metabolism Receptors, CXCR4 / genetics Receptors, CXCR4 / metabolism Virus Internalization* |
| Altmetric score |
オルトメトリクス指標項目
|
| Total number of mentions | 0 |
| Resource | |
| Human and Animal Cells | GM95(RCB1026) |