RRC ID 69860
著者 Rattanawong K, Koiso N, Toda E, Kinoshita A, Tanaka M, Tsuji H, Okamoto T.
タイトル Regulatory functions of ROS dynamics via glutathione metabolism and glutathione peroxidase activity in developing rice zygote.
ジャーナル Plant J
Abstract Reactive oxygen species (ROS) play essential roles in plant development and environmental stress responses. In this study, ROS dynamics, the glutathione redox status, the expression and subcellular localization of glutathione peroxidases (GPXs), and the effects of inhibitors of ROS-mediated metabolism were investigated along with fertilization and early zygotic embryogenesis in rice (Oryza sativa). Zygotes and early embryos exhibited developmental arrest upon inhibition of ROS production. Egg cells accumulated high ROS levels, and, after fertilization, intracellular ROS levels progressively declined in zygotes in which de novo expression of GPX1 and 3 was observed through upregulation of the genes. In addition to inhibition of GPX activity, depletion of glutathione impeded early embryonic development and led to failure of the zygote to appropriately decrease H2 O2 levels. Moreover, through monitoring of the glutathione redox status, the developing zygotes exhibited a progressive glutathione oxidation, which became extremely delayed under inhibited GPX activity. Our results provide insights into the importance of ROS dynamics, GPX antioxidant activity, and glutathione redox metabolism during zygotic/embryonic development.
巻・号 108(4)
ページ 1097-1115
公開日 2021-11-1
DOI 10.1111/tpj.15497
PMID 34538012
PMC PMC9293154
MeSH Antioxidants / metabolism* Glutathione / metabolism* Glutathione Peroxidase / genetics Glutathione Peroxidase / metabolism* Oryza / enzymology Oryza / genetics Oryza / growth & development Oryza / physiology* Oxidation-Reduction Plant Proteins / genetics Plant Proteins / metabolism Reactive Oxygen Species / metabolism* Stress, Physiological Zygote
IF 6.141
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 rpc00031