RRC ID 69890
Author Waheed H, Farrukh S, Hussain A, Mukhtar A, Mubashir M, Saqib S, Ullah S, Peter AP, Khoo KS, Show PL.
Title Green synthesized nano-cellulose polyethylene imine-based biological membrane.
Journal Food Chem Toxicol
Abstract In hemodialysis process, membrane serves as a barrier between blood and the dialysate. The barrier when contacted by blood accompanied activation of coagulation, immunity, and cellular passageways. In the recent years, hemodialysis membrane's biocompatibility has become a issue which leads to reduce the performance during the separation process. In previous work, we developed and evaluated a cellulose-based membrane blended with polyaziridine or polyetyleneimine in formic acid for hydrophilicity, pure water flux, surface morphology, and permeation efficiency. Biocompatibility was accessed, by conducting cellular viability and cellular attachments tests. In this study, the membrane compared to a non-treated control, and cell viability revealed active and growing cell cultures after 14 days. During the cellular attachment experiment, cell cultures attached to the fabricated membrane simulated the formation of cell junctions, proving that the membrane is non-toxic and biocompatible. CA + PEI + FA membrane tested with a blood mimic fluid having density identical to renal patient's blood. The BSA concentration in the feed solution was the same as that in the blood of the renal patient. The results revealed that the CA + PEI + FA membrane was able to reject 99% bovine serum albumin (BSA) and 69.6% urea. Therefore, from biocompatibility and blood mimic fluid testing, it is confirmed that the CA + PEI + FA membrane is the finest implant for dialysis applications.
Volume 160
Pages 112773
Published 2022-2-1
DOI 10.1016/j.fct.2021.112773
PII S0278-6915(21)00806-1
PMID 34953965
MeSH Biocompatible Materials / chemical synthesis* Biocompatible Materials / chemistry Cell Adhesion Cell Survival Cellulose / analogs & derivatives* Cellulose / chemical synthesis Cellulose / chemistry Formates / chemistry Green Chemistry Technology Humans Hydrophobic and Hydrophilic Interactions Membranes, Artificial Nanoparticles / chemistry* Polyethyleneimine / analogs & derivatives* Polyethyleneimine / chemical synthesis Polyethyleneimine / chemistry Renal Dialysis / instrumentation*
IF 4.679
Human and Animal Cells MC3T3-E1(RCB1126)