| RRC ID |
6991
|
| 著者 |
Han J, Hou J, Liu H, Cai S, Feng B, Zhou J, Xiang H.
|
| タイトル |
Wide distribution among halophilic archaea of a novel polyhydroxyalkanoate synthase subtype with homology to bacterial type III synthases.
|
| ジャーナル |
Appl Environ Microbiol
|
| Abstract |
Polyhydroxyalkanoates (PHAs) are accumulated as intracellular carbon and energy storage polymers by various bacteria and a few haloarchaea. In this study, 28 strains belonging to 15 genera in the family Halobacteriaceae were investigated with respect to their ability to synthesize PHAs and the types of their PHA synthases. Fermentation results showed that 18 strains from 12 genera could synthesize polyhydroxybutyrate (PHB) or poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). For most of these haloarchaea, selected regions of the phaE and phaC genes encoding PHA synthases (type III) were cloned via PCR with consensus-degenerate hybrid oligonucleotide primers (CODEHOPs) and were sequenced. The PHA synthases were also examined by Western blotting using haloarchaeal Haloarcula marismortui PhaC (PhaC(Hm)) antisera. Phylogenetic analysis showed that the type III PHA synthases from species of the Halobacteriaceae and the Bacteria domain clustered separately. Comparison of their amino acid sequences revealed that haloarchaeal PHA synthases differed greatly in both molecular weight and certain conserved motifs. The longer C terminus of haloarchaeal PhaC was found to be indispensable for its enzymatic activity, and two additional amino acid residues (C143 and C190) of PhaC(Hm) were proved to be important for its in vivo function. Thus, we conclude that a novel subtype (IIIA) of type III PHA synthase with unique features that distinguish it from the bacterial subtype (IIIB) is widely distributed in haloarchaea and appears to be involved in PHA biosynthesis.
|
| 巻・号 |
76(23)
|
| ページ |
7811-9
|
| 公開日 |
2010-12-1
|
| DOI |
10.1128/AEM.01117-10
|
| PII |
AEM.01117-10
|
| PMID |
20889776
|
| PMC |
PMC2988587
|
| MeSH |
Acyltransferases / genetics*
Acyltransferases / metabolism*
Blotting, Western
Cluster Analysis
DNA, Archaeal / chemistry
DNA, Archaeal / genetics
Fermentation
Haloarcula marismortui / enzymology
Haloarcula marismortui / immunology
Halobacteriaceae / enzymology*
Halobacteriaceae / genetics*
Hydroxybutyrates / metabolism
Molecular Sequence Data
Phylogeny
Polyesters / metabolism
Sequence Analysis, DNA
Sequence Homology
|
| IF |
4.016
|
| 引用数 |
50
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 8980
JCM 10476 |
