論文 - 詳細
RRC ID | 6992 |
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著者 | Shi P, Yao G, Yang P, Li N, Luo H, Bai Y, Wang Y, Yao B. |
タイトル | Cloning, characterization, and antifungal activity of an endo-1,3-beta-D: -glucanase from Streptomyces sp. S27. |
ジャーナル | Appl Microbiol Biotechnol |
Abstract |
An endo-1,3-beta-D: -glucanase gene, designated as bglS27, was cloned from Streptomyces sp. S27 and successfully expressed in Escherichia coli BL21 (DE3). The full-length gene contains 1,362 bp and encodes a protein of 453 amino acids with a calculated molecular mass of 42.7 kDa. The encoded protein comprises a catalytic module of glycosyl hydrolase family 16, a short glycine linker region, and a family 13 carbohydrate-binding module. The purified recombinant enzyme (BglS27) showed optimal activity at 65 degrees C and pH 5.5 and preferentially catalyzed the hydrolysis of glucans with a beta-1,3-linkage using an endolytic mode of action. The specific activity and K(m) value of BglS27 for laminarin were 236.0 U mg(-1) and 1.89 mg ml(-1), respectively. In antifungal assay, BglS27 had the ability to inhibit the growth of phytopathogenic fungi Rhizoctonic solani and Fusarium oxysporum and some mycotoxin-producing fungi Fusarium crookwellense and Paecilomyces variotii. These favorable properties make BglS27 a good candidate for utilization in biotechnological applications such as plant protection, feed, and food preservation. |
巻・号 | 85(5) |
ページ | 1483-90 |
公開日 | 2010-2-1 |
DOI | 10.1007/s00253-009-2187-1 |
PMID | 19697016 |
MeSH | Amino Acid Sequence Antifungal Agents / chemistry Antifungal Agents / pharmacology* Bacterial Proteins / chemistry Bacterial Proteins / genetics Bacterial Proteins / metabolism Bacterial Proteins / pharmacology Base Sequence Cloning, Molecular* DNA, Bacterial / genetics Escherichia coli / genetics Escherichia coli / metabolism Fungi / drug effects* Genes, Bacterial Glucan Endo-1,3-beta-D-Glucosidase / chemistry Glucan Endo-1,3-beta-D-Glucosidase / genetics Glucan Endo-1,3-beta-D-Glucosidase / metabolism* Glucan Endo-1,3-beta-D-Glucosidase / pharmacology* Glucans / metabolism Hydrolysis Molecular Sequence Data Polysaccharides / metabolism Recombinant Proteins / chemistry Recombinant Proteins / metabolism Recombinant Proteins / pharmacology Sequence Alignment Streptomyces / enzymology* Streptomyces / genetics Streptomyces / metabolism Substrate Specificity |
IF | 3.53 |
引用数 | 29 |
WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
リソース情報 | |
一般微生物 | JCM 9874 |