論文 - 詳細
| RRC ID | 69996 |
|---|---|
| 著者 | Inoue M, Takeuchi A, Manita S, Horigane SI, Sakamoto M, Kawakami R, Yamaguchi K, Otomo K, Yokoyama H, Kim R, Yokoyama T, Takemoto-Kimura S, Abe M, Okamura M, Kondo Y, Quirin S, Ramakrishnan C, Imamura T, Sakimura K, Nemoto T, Kano M, Fujii H, Deisseroth K, Kitamura K, Bito H. |
| タイトル | Rational Engineering of XCaMPs, a Multicolor GECI Suite for In Vivo Imaging of Complex Brain Circuit Dynamics. |
| ジャーナル | Cell |
| Abstract |
To decipher dynamic brain information processing, current genetically encoded calcium indicators (GECIs) are limited in single action potential (AP) detection speed, combinatorial spectral compatibility, and two-photon imaging depth. To address this, here, we rationally engineered a next-generation quadricolor GECI suite, XCaMPs. Single AP detection was achieved within 3-10 ms of spike onset, enabling measurements of fast-spike trains in parvalbumin (PV)-positive interneurons in the barrel cortex in vivo and recording three distinct (two inhibitory and one excitatory) ensembles during pre-motion activity in freely moving mice. In vivo paired recording of pre- and postsynaptic firing revealed spatiotemporal constraints of dendritic inhibition in layer 1 in vivo, between axons of somatostatin (SST)-positive interneurons and apical tufts dendrites of excitatory pyramidal neurons. Finally, non-invasive, subcortical imaging using red XCaMP-R uncovered somatosensation-evoked persistent activity in hippocampal CA1 neurons. Thus, the XCaMPs offer a critical enhancement of solution space in studies of complex neuronal circuit dynamics. VIDEO ABSTRACT. |
| 巻・号 | 177(5) |
| ページ | 1346-1360.e24 |
| 公開日 | 2019-5-16 |
| DOI | 10.1016/j.cell.2019.04.007 |
| PII | S0092-8674(19)30393-9 |
| PMID | 31080068 |
| MeSH | Action Potentials / physiology* Animals Axons / metabolism* Cerebral Cortex / cytology Cerebral Cortex / metabolism* Female Hippocampus / cytology Hippocampus / metabolism* Interneurons / cytology Interneurons / metabolism* Mice Mice, Transgenic Pyramidal Cells / cytology Pyramidal Cells / metabolism* Rats Rats, Sprague-Dawley |
| IF | 38.637 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 269 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pBS-CaMKIIpro-XCaMP-Gf-WPRE (RDB19470) pBS-CaMKIIpro-XCaMP-G-WPRE (RDB19471) pBS-CaMKIIpro-XCaMP-Y-WPRE (RDB19472) pBS-CaMKIIpro-XCaMP-B-WPRE (RDB19473) pBS-CaMKIIpro-XCaMP-R-WPRE (RDB19474) |