Reference - Detail
| RRC ID | 70000 |
|---|---|
| Author | Kawaguchi A, Nagata K. |
| Title | De novo replication of the influenza virus RNA genome is regulated by DNA replicative helicase, MCM. |
| Journal | EMBO J |
| Abstract |
By dissecting and reconstituting a cell-free influenza virus genome replication system, we have purified and identified the minichromosome maintenance (MCM) complex, which is thought to be a DNA replicative helicase, as one of the host factors that regulate the virus genome replication. MCM interacted with the PA subunit of the viral RNA-dependent RNA polymerase that is found to be involved in the replication genetically. The virus genome replication was decreased in MCM2 knockdown cells. The viral polymerase appeared to be a nonproductive complex, that is, it was capable of initiating replication but produced only abortive short RNA chains. MCM stimulated de novo-initiated replication reaction by stabilizing a replication complex during its transition from initiation to elongation. Based on the findings, including the result that the MCM-mediated RNA replication reaction was competed with exogenously added RNA, we propose that MCM functions as a scaffold between the nascent RNA chains and the viral polymerase. |
| Volume | 26(21) |
| Pages | 4566-75 |
| Published | 2007-10-31 |
| DOI | 10.1038/sj.emboj.7601881 |
| PII | 7601881 |
| PMID | 17932485 |
| PMC | PMC2063485 |
| MeSH | Cell-Free System DNA Replication DNA-Directed RNA Polymerases / chemistry DNA-Directed RNA Polymerases / genetics Genome, Viral* Hydrolysis Minichromosome Maintenance 1 Protein / genetics Models, Genetic Nucleic Acid Conformation Orthomyxoviridae / genetics* RNA / chemistry RNA, Viral / genetics* Viral Proteins / chemistry Virus Replication |
| IF | 9.889 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 6 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pCAGGS-PB2cFLAG (RDB19457) pCAGGS-NP (RDB19456) pCAGGS-PA (RDB19455) pCAGGS-PB2 (RDB19454) pCAGGS-PB1 (RDB19453) |