論文 - 詳細
| RRC ID | 7004 |
|---|---|
| 著者 | Tsuruta T, Inoue R, Iwanaga T, Hara H, Yajima T. |
| タイトル | Development of a method for the identification of S-IgA-coated bacterial composition in mouse and human feces. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
Some commensal intestinal bacteria in humans and mice are coated with secretory immunoglobulin A (S-IgA). It has been suggested that the S-IgA coating of commensal bacteria does not occur at random and thus identification of S-IgA-coated bacterial genera/species should help in elucidating the interaction between S-IgA and commensal intestinal bacteria, but no method of identifying the genera/species of S-IgA-coated bacteria has been established. To identify S-IgA-coated bacterial composition, we developed a method combining immunohistochemical detection of S-IgA and subsequent 16S rRNA targeted fluorescence in situ hybridization (FISH) analysis. Human and mouse fecal S-IgA coated bacterial composition was evaluated by this newly developed method with 10 frequently-used FISH probes. Fecal S-IgA-coated bacterial composition was successfully analyzed by this method, and this suggests that Enterobacteriaceae is preferably coated with S-IgA, whereas Bacteroides/Prevotella and Lactobacillus/Enterococcus groups appear to be poorly coated with S-IgA. |
| 巻・号 | 74(5) |
| ページ | 968-73 |
| 公開日 | 2010-1-1 |
| DOI | 10.1271/bbb.90801 |
| PII | JST.JSTAGE/bbb/90801 |
| PMID | 20460734 |
| MeSH | Adult Animals Bacteria / classification* Bacteria / genetics Bacteria / isolation & purification Bacteria / metabolism* Bacterial Typing Techniques / methods* Base Sequence Feces / microbiology* Female Glass Humans Immunoglobulin A, Secretory / metabolism* In Situ Hybridization, Fluorescence Mice Reproducibility of Results |
| IF | 1.516 |
| 引用数 | 15 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 5827 JCM 1395 JCM 11662 JCM 8129 JCM 14654 JCM 9981 JCM 1772 |