論文 - 詳細
| RRC ID | 7006 |
|---|---|
| 著者 | Moe KK, Yano T, Misumi K, Kubota C, Yamazaki W, Muguruma M, Misawa N. |
| タイトル | Analysis of the IgG immune response to Treponema phagedenis-like spirochetes in individual dairy cattle with papillomatous digital dermatitis. |
| ジャーナル | Clin Vaccine Immunol |
| Abstract |
Papillomatous digital dermatitis (PDD) is a major infectious disease of the foot skin in dairy cattle. Treponema phagedenis-like spirochetes have been consistently detected in PDD lesions, and antibodies against these organisms have been demonstrated in affected cattle. However, little is known about the dominant antigens recognized by the immune system of affected cattle. Here, we investigated the IgG immune response to T. phagedenis-like isolates by Western blotting with different sera using whole-cell lysates and extracted glycolipid from 18 and 8 isolates, respectively, including those from different cattle on the same or different farms, isolates from different lesions affecting a single cow, and different isolates from the same lesion affecting a single cow. The reactivity of sera in Western blot assays revealed different banding patterns or showed no bands, suggesting that considerable antigenic variations, including glycolipid, may exist among the isolates, even in those from single individuals. With use of a total of 151 serum samples collected from three groups of cattle, i.e., PDD-positive cows on PDD-positive farms (group A), PDD-negative cows on PDD-positive farms (group B), and cows on PDD-free farms (group C), the levels of IgG antibodies against four T. phagedenis-like isolates were measured by enzyme-linked immunosorbent assay (ELISA). The optical density in groups A and B was significantly higher than that in group C, even though the value varied among the antigens used. Therefore, combinations of multiple Treponema species should be used for serological analysis and the development of a suitable vaccine because of antigenic variations. |
| 巻・号 | 17(3) |
| ページ | 376-83 |
| 公開日 | 2010-3-1 |
| DOI | 10.1128/CVI.00464-09 |
| PII | CVI.00464-09 |
| PMID | 20107009 |
| PMC | PMC2837959 |
| MeSH | Animals Antibodies, Bacterial / blood Antigens, Bacterial / immunology Blotting, Western Cattle Dermatitis / immunology* Dermatitis / microbiology Dermatitis / veterinary* Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Foot Diseases / immunology Foot Diseases / microbiology Foot Diseases / veterinary* Hoof and Claw Immunoglobulin G / blood* Immunoglobulin G / immunology Treponema / genetics Treponema / immunology Treponema / isolation & purification Treponemal Infections / blood Treponemal Infections / immunology* |
| IF | 3.233 |
| 引用数 | 12 |
| WOS 分野 | INFECTIOUS DISEASES MICROBIOLOGY IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 8225 |