RRC ID 70102
Author Okada S, Fukai Y, Tanoue Y, Nasser H, Fukuda T, Ikeda T, Saitoh H.
Title Basic structure and cytocompatibility of giant membrane vesicles derived from paraformaldehyde-exposed human cells.
Journal J Biochem
Abstract Exposure of cultured mammalian cells to paraformaldehyde (PFA) is an effective approach to induce membrane blebs, which is followed by their detachment from the cellular cortex to yield giant membrane vesicles in extracellular spaces. Although PFA-induced giant vesicles have attracted significant interest in the field of cell membrane dynamics, their biochemical components and cytocompatibility remain largely unknown. In this report, we exposed human cervical cancer HeLa cells to PFA under metal-free buffer conditions to produce giant vesicles. We analyzed the components and structure of the purified PFA-induced giant vesicles. Co-culturing PFA-induced giant vesicles with exponentially growing HeLa cells resulted in docking of a significant number of the giant vesicles to the cell surface with seemingly no cytotoxicity. Intriguingly, we found that pre-treatment of HeLa cells with peptide-N-glycosidase and neuraminidase was effective in facilitating cellular uptake of constituents residing inside the vesicles. The results revealed further details about the effect of PFA on cell membranes and provide insights for studying the interaction between PFA-induced giant vesicles and cultured cells.
Volume 171(3)
Pages 339-347
Published 2022-3-3
DOI 10.1093/jb/mvab144
PII 6464658
PMID 34928331
MeSH Animals Blister* / metabolism Cell Membrane / metabolism Formaldehyde* / analysis Formaldehyde* / metabolism Formaldehyde* / pharmacology HeLa Cells Humans Polymers / metabolism Polymers / pharmacology
IF 2.476
Human and Animal Cells HeLa