RRC ID 70162
Author Chang W, Pedroni A, Bertuzzi M, Kizil C, Simon A, Ampatzis K.
Title Locomotion dependent neuron-glia interactions control neurogenesis and regeneration in the adult zebrafish spinal cord.
Journal Nat Commun
Abstract Physical exercise stimulates adult neurogenesis, yet the underlying mechanisms remain poorly understood. A fundamental component of the innate neuroregenerative capacity of zebrafish is the proliferative and neurogenic ability of the neural stem/progenitor cells. Here, we show that in the intact spinal cord, this plasticity response can be activated by physical exercise by demonstrating that the cholinergic neurotransmission from spinal locomotor neurons activates spinal neural stem/progenitor cells, leading to neurogenesis in the adult zebrafish. We also show that GABA acts in a non-synaptic fashion to maintain neural stem/progenitor cell quiescence in the spinal cord and that training-induced activation of neurogenesis requires a reduction of GABAA receptors. Furthermore, both pharmacological stimulation of cholinergic receptors, as well as interference with GABAergic signaling, promote functional recovery after spinal cord injury. Our findings provide a model for locomotor networks’ activity-dependent neurogenesis during homeostasis and regeneration in the adult zebrafish spinal cord.
Volume 12(1)
Pages 4857
Published 2021-8-11
DOI 10.1038/s41467-021-25052-1
PII 10.1038/s41467-021-25052-1
PMID 34381039
PMC PMC8357999
MeSH Animals Interneurons / metabolism Locomotion* Neural Stem Cells / cytology Neural Stem Cells / metabolism Neurogenesis Neuroglia / metabolism* Neurons / metabolism* Physical Conditioning, Animal Receptors, Cholinergic / metabolism Receptors, GABA-A / metabolism Recovery of Function Spinal Cord / cytology Spinal Cord / growth & development* Spinal Cord / physiology Synaptic Transmission Zebrafish gamma-Aminobutyric Acid / metabolism
IF 12.121
Zebrafish Tg(chx10:GFP)