RRC ID 70169
Author Tomihara S, Oka Y, Kanda S.
Title Establishment of open-source semi-automated behavioral analysis system and quantification of the difference of sexual motivation between laboratory and wild strains.
Journal Sci Rep
Abstract Behavioral analysis plays an important role in wide variety of biological studies, but behavioral recordings often tend to be laborious and are associated with inevitable human-errors. It also takes much time to perform manual behavioral analyses while replaying the videos. On the other hand, presently available automated recording/analysis systems are often specialized for certain types of behavior of specific animals. Here, we established an open-source behavioral recording system using Raspberry Pi, which automatically performs video-recording and systematic file-sorting, and the behavioral recording can be performed more efficiently, without unintentional human operational errors. We also developed an Excel macro that enables us to easily perform behavioral annotation with simple manipulation. Thus, we succeeded in developing an analysis suite that mitigates human tasks and thus reduces human errors. By using this suite, we analyzed the sexual behavior of a laboratory and a wild medaka strain and found a difference in sexual motivation presumably resulting from domestication.
Volume 11(1)
Pages 10894
Published 2021-5-25
DOI 10.1038/s41598-021-90225-3
PII 10.1038/s41598-021-90225-3
PMID 34035352
PMC PMC8149402
MeSH Animals Automation, Laboratory Behavior, Animal / physiology Motivation* Oryzias / physiology* Sexual Behavior* User-Computer Interface Video Recording
IF 3.998
Medaka Kiyosu (WS1177)