| RRC ID |
7016
|
| 著者 |
Cui HL, Sun FF, Gao X, Dong Y, Xu XW, Zhou YG, Liu HC, Oren A, Zhou PJ.
|
| タイトル |
Haladaptatus litoreus sp. nov., an extremely halophilic archaeon from a marine solar saltern, and emended description of the genus Haladaptatus.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
Two extremely halophilic archaea, strains RO1-28(T) and RO1-22, were isolated from a marine solar saltern in Jiangsu, China. Both strains required at least 0.05 M Mg(2+) and 1.7 M NaCl for growth. They were able to grow over a pH range of 6.0-8.5 and a temperature range of 25-55 degrees C, with optimal pH of 7.0 and optimal temperature of 37-40 degrees C. Based on 16S rRNA gene sequence analysis, strains RO1-28(T) and RO1-22 were closely related to Haladaptatus paucihalophilus, the single species of the genus Haladaptatus, with similarities of 94.0-95.2 %. The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and three glycolipids chromatographically identical to the glycolipids of Haladaptatus paucihalophilus JCM 13897(T). Both strains RO1-28(T) and RO1-22 had a DNA G+C content of 54.0 mol% (HPLC). The DNA-DNA hybridization value between the two strains was more than 70 % (92 %) and both strains showed low levels of DNA-DNA relatedness (32 % and 33 %) with Haladaptatus paucihalophilus JCM 13897(T). It was concluded that strains RO1-28(T) and RO1-22 represent a novel species of the genus Haladaptatus, for which the name Haladaptatus litoreus sp. nov. is proposed. The type strain is RO1-28(T) (=CGMCC 1.7737(T) =JCM 15771(T)).
|
| 巻・号 |
60(Pt 5)
|
| ページ |
1085-1089
|
| 公開日 |
2010-5-1
|
| DOI |
10.1099/ijs.0.015933-0
|
| PMID |
19666806
|
| MeSH |
Base Composition
China
DNA, Archaeal / analysis
DNA, Ribosomal / analysis
Genes, rRNA
Geologic Sediments / microbiology*
Halobacteriaceae / chemistry
Halobacteriaceae / classification*
Halobacteriaceae / genetics
Halobacteriaceae / isolation & purification*
Lipids / analysis
Molecular Sequence Data
Nucleic Acid Hybridization
Phenotype
RNA, Ribosomal, 16S / genetics
Seawater / microbiology*
Sequence Analysis, DNA
Sodium Chloride*
Species Specificity
Temperature
|
| IF |
2.415
|
| 引用数 |
13
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 15771
JCM 13897
JCM 14081 |
