| RRC ID |
7021
|
| Author |
Burns DG, Janssen PH, Itoh T, Kamekura M, Echigo A, Dyall-Smith ML.
|
| Title |
Halonotius pteroides gen. nov., sp. nov., an extremely halophilic archaeon recovered from a saltern crystallizer.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
Strains 1.15.5(T), 2.27.5, 5.24.4 and 6.14.5 were isolated from a solar saltern. They have flattened, rod-shaped cells and are aerobic, extremely halophilic members of the domain Archaea and family Halobacteriaceae. Cells stained Gram-negative and grew optimally in media around neutral pH and containing 20-24 % (w/v) (strains 1.15.5(T) and 2.27.5) or 22-24 % (w/v) (5.24.4 and 6.14.5) salts. Mg(2+) was not required. The DNA G+C contents of these isolates were all close to 58 mol%, and DNA-DNA cross-hybridization showed a mean relatedness of 77 %. Their 16S rRNA gene sequences differed by no more than 1.6 % from each other. Phylogenetic tree reconstructions with other recognized members of the Halobacteriaceae indicated that they formed a distinct clade, with the closest relative being Halorubrum saccharovorum (86.6-87.6 % 16S rRNA gene sequence similarity to the type strain). The only major polar lipid of all four isolates was the sulfated diglycosyl diether lipid S-DGD-1. By phase-contrast microscopy, the long, flattened cells of these strains often displayed a 'wing-like' shape. The phenotypic and phylogenetic data support the placement of these isolates into a novel species in a new genus within the Halobacteriaceae, for which we propose the name Halonotius pteroides gen. nov., sp. nov. The type strain of Halonotius pteroides is 1.15.5(T) (=JCM 14355(T) =CECT 7525(T) =DSM 18729(T)), with the additional reference strains 2.27.5 (=JCM 14356 =DSM 18671), 5.24.4 (=JCM 14357 =DSM 18673) and 6.14.5 (=JCM 14358 =DSM 18692).
|
| Volume |
60(Pt 5)
|
| Pages |
1196-1199
|
| Published |
2010-5-1
|
| DOI |
10.1099/ijs.0.010017-0
|
| PMID |
19667389
|
| MeSH |
Base Composition
Crystallization
DNA, Archaeal / analysis
DNA, Ribosomal / analysis
Genes, rRNA
Genotype
Halobacteriaceae / classification*
Halobacteriaceae / genetics
Halobacteriaceae / isolation & purification
Halobacteriaceae / physiology
Molecular Sequence Data
Nucleic Acid Hybridization
Phenotype
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sodium Chloride*
Species Specificity
Water Microbiology*
|
| IF |
2.415
|
| Times Cited |
27
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 14355
JCM 14356
JCM 14357
JCM 14358
JCM 10706
JCM 9101
JCM 9738
JCM 11627 |
