Reference - Detail
| RRC ID | 70262 |
|---|---|
| Author | Itaya M. |
| Title | Genetic transfer of large DNA inserts to designated loci of the Bacillus subtilis 168 genome. |
| Journal | J Bacteriol |
| Abstract |
It was found that contiguous DNA segments of up to 50 kb can be transferred between Bacillus subtilis genomes when a sufficient length of the flanking genomic region is provided for homologous recombination, although the efficiency of transfer was reduced as the insert size increased. Inserts were translocated to different loci, where appropriate integration sites were created. |
| Volume | 181(3) |
| Pages | 1045-8 |
| Published | 1999-2-1 |
| DOI | 10.1128/JB.181.3.1045-1048.1999 |
| PMID | 9922275 |
| PMC | PMC93478 |
| MeSH | Bacillus subtilis / genetics* Bacteriophage lambda / genetics DNA Probes DNA, Viral / genetics Escherichia coli / genetics Gene Transfer Techniques* Genome, Bacterial* Mutagenesis, Insertional / methods* Plasmids Recombination, Genetic* Restriction Mapping |
| IF | 3.006 |
| Resource | |
| Prokaryotes B. subtilis | MBS959 MBS960 MBS961 MBS962 MBS963 MBS964 MBS965 MBS966 MBS967 MBS968 MBS969 MBS971 MBS972 MBS973 |