RRC ID 70326
Author Smirnova A, Mentor A, Ranefall P, Bornehag CG, Brunström B, Mattsson A, Jönsson M.
Title Increased apoptosis, reduced Wnt/β-catenin signaling, and altered tail development in zebrafish embryos exposed to a human-relevant chemical mixture.
Journal Chemosphere
Abstract A wide variety of anthropogenic chemicals is detected in humans and wildlife and the health effects of various chemical exposures are not well understood. Early life stages are generally the most susceptible to chemical disruption and developmental exposure can cause disease in adulthood, but the mechanistic understanding of such effects is poor. Within the EU project EDC-MixRisk, a chemical mixture (Mixture G) was identified in the Swedish pregnancy cohort SELMA by the inverse association between levels in women at around gestational week ten with birth weight of their children. This mixture was composed of mono-ethyl phthalate, mono-butyl phthalate, mono-benzyl phthalate, mono-ethylhexyl phthalate, mono-isononyl phthalate, triclosan, perfluorohexane sulfonate, perfluorooctanoic acid, and perfluorooctane sulfonate. In a series of experimental studies, we characterized effects of Mixture G on early development in zebrafish models. Here, we studied apoptosis and Wnt/β-catenin signaling which are two evolutionarily conserved signaling pathways of crucial importance during development. We determined effects on apoptosis by measuring TUNEL staining, caspase-3 activity, and acridine orange staining in wildtype zebrafish embryos, while Wnt/β-catenin signaling was assayed using a transgenic line expressing an EGFP reporter at β-catenin-regulated promoters. We found that Mixture G increased apoptosis, suppressed Wnt/β-catenin signaling in the caudal fin, and altered the shape of the caudal fin at water concentrations only 20-100 times higher than the geometric mean serum concentration in the human cohort. These findings call for awareness that pollutant mixtures like mixture G may interfere with a variety of developmental processes, possibly resulting in adverse health effects.
Volume 264(Pt 1)
Pages 128467
Published 2021-2-1
DOI 10.1016/j.chemosphere.2020.128467
PII S0045-6535(20)32662-X
PMID 33032226
MeSH Adult Animals Apoptosis Child Female Humans Pregnancy Wnt Signaling Pathway Zebrafish* / genetics Zebrafish* / metabolism Zebrafish Proteins beta Catenin* / genetics beta Catenin* / metabolism
IF 5.778
Zebrafish Tg(6xTcf/LefBS-miniP:d2EGFP)isi04