RRC ID 70415
著者 Sakata KT, Hashii K, Yoshizawa K, Tahara YO, Yae K, Tsuda R, Tanaka N, Maeda T, Miyata M, Tabuchi M.
タイトル Coordinated regulation of TORC2 signaling by MCC/eisosome-associated proteins, Pil1 and tetraspan membrane proteins during the stress response.
ジャーナル Mol Microbiol
Abstract MCCs are linear invaginations of the yeast plasma membrane that form stable membrane microdomains. Although over 20 proteins are localized in the MCCs, it is not well understood how these proteins coordinately maintain normal MCC function. Pil1 is a core eisosome protein and is responsible for MCC-invaginated structures. In addition, six-tetraspan membrane proteins (6-Tsp) are localized in the MCCs and classified into two families, the Sur7 family and Nce102 family. To understand the coordinated function of these MCC proteins, single and multiple deletion mutants of Pil1 and 6-Tsp were generated and their MCC structure and growth under various stresses were investigated. Genetic interaction analysis revealed that the Sur7 family and Nce102 function in stress tolerance and normal eisosome assembly, respectively, by cooperating with Pil1. To further understand the role of MCCs/eisosomes in stress tolerance, we screened for suppressor mutants using the SDS-sensitive phenotype of pil1Δ 6-tspΔ cells. This revealed that SDS sensitivity is caused by hyperactivation of Tor kinase complex 2 (TORC2)-Ypk1 signaling. Interestingly, inhibition of sphingolipid metabolism, a well-known downstream pathway of TORC2-Ypk1 signaling, did not rescue the SDS-sensitivity of pil1Δ 6-tspΔ cells. These results suggest that Pil1 and 6-Tsp cooperatively regulate TORC2 signaling during the stress response.
巻・号 117(5)
ページ 1227-1244
公開日 2022-5-1
DOI 10.1111/mmi.14903
PMID 35383382
MeSH Cell Membrane / metabolism Mechanistic Target of Rapamycin Complex 2 / metabolism Membrane Microdomains / metabolism Membrane Proteins / genetics Membrane Proteins / metabolism Phosphoproteins / metabolism Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins* / metabolism
IF 3.418
リソース情報
酵母 NBRP Yeast