RRC ID 70447
Author Zhao S, Feng S, Tian Y, Wen Z.
Title Hemogenic and aortic endothelium arise from a common hemogenic angioblast precursor and are specified by the Etv2 dosage.
Journal Proc Natl Acad Sci U S A
Abstract SignificanceHematopoietic stem cells (HSCs) are generated from specialized endothelial cells, called hemogenic endothelial cells (HECs). It has been debated whether HECs and non-HSC-forming conventional endothelial cells (cECs) arise from a common precursor or represent distinct lineages. Moreover, the molecular basis underlying their distinct fate determination is poorly understood. We use photoconvertible labeling, time-lapse imaging, and single-cell RNA-sequencing analysis to trace the lineage of HECs. We discovered that HECs and cECs arise from a common hemogenic angioblast precursor, and their distinct fate is determined by high or low dosage of Etv2, respectively. Our results illuminate the lineage origin and a mechanism on the fate determination of HECs, which may enhance the understanding on the ontogeny of HECs in vertebrates.
Volume 119(13)
Pages e2119051119
Published 2022-3-29
DOI 10.1073/pnas.2119051119
PMID 35333649
PMC PMC9060440
MeSH Animals Cell Differentiation Endothelium, Vascular Hemangioblasts* Hematopoiesis*
IF 9.412
Zebrafish Tg(kdrl:NLS-Eos)ncv6Tg