RRC ID 70516
Author Yamada Y.
Title RPD3 and UME6 are involved in the activation of PDR5 transcription and pleiotropic drug resistance in ρ0 cells of Saccharomyces cerevisiae.
Journal BMC Microbiol
Abstract BACKGROUND:In Saccharomyces cerevisiae, the retrograde signalling pathway is activated in ρ0/- cells, which lack mitochondrial DNA. Within this pathway, the activation of the transcription factor Pdr3 induces transcription of the ATP-binding cassette (ABC) transporter gene, PDR5, and causes pleiotropic drug resistance (PDR). Although a histone deacetylase, Rpd3, is also required for cycloheximide resistance in ρ0/- cells, it is currently unknown whether Rpd3 and its DNA binding partners, Ume6 and Ash1, are involved in the activation of PDR5 transcription and PDR in ρ0/- cells. This study investigated the roles of RPD3, UME6, and ASH1 in the activation of PDR5 transcription and PDR by retrograde signalling in ρ0 cells.
RESULTS:ρ0 cells in the rpd3∆ and ume6∆ strains, with the exception of the ash1∆ strain, were sensitive to fluconazole and cycloheximide. The PDR5 mRNA levels in ρ0 cells of the rpd3∆ and ume6∆ strains were significantly reduced compared to the wild-type and ash1∆ strain. Transcriptional expression of PDR5 was reduced in cycloheximide-exposed and unexposed ρ0 cells of the ume6∆ strain; the transcriptional positive response of PDR5 to cycloheximide exposure was also impaired in this strain.
CONCLUSIONS:RPD3 and UME6 are responsible for enhanced PDR5 mRNA levels and PDR by retrograde signalling in ρ0 cells of S. cerevisiae.
Volume 21(1)
Pages 311
Published 2021-11-9
DOI 10.1186/s12866-021-02373-1
PII 10.1186/s12866-021-02373-1
PMID 34753419
PMC PMC8576940
MeSH ATP-Binding Cassette Transporters / genetics* ATP-Binding Cassette Transporters / metabolism Antifungal Agents / pharmacology* Cycloheximide / pharmacology Fluconazole / pharmacology Gene Expression Regulation, Fungal* / drug effects Histone Deacetylases / genetics Histone Deacetylases / metabolism* Mitochondria / drug effects Mitochondria / genetics Mitochondria / metabolism Repressor Proteins / genetics Repressor Proteins / metabolism* Saccharomyces cerevisiae / drug effects Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins / genetics* Saccharomyces cerevisiae Proteins / metabolism* Transcriptional Activation / drug effects
IF 2.989
Yeast BY4848