論文 - 詳細
| RRC ID | 70516 |
|---|---|
| 著者 | Yamada Y. |
| タイトル | RPD3 and UME6 are involved in the activation of PDR5 transcription and pleiotropic drug resistance in ρ0 cells of Saccharomyces cerevisiae. |
| ジャーナル | BMC Microbiol |
| Abstract |
BACKGROUND:In Saccharomyces cerevisiae, the retrograde signalling pathway is activated in ρ0/- cells, which lack mitochondrial DNA. Within this pathway, the activation of the transcription factor Pdr3 induces transcription of the ATP-binding cassette (ABC) transporter gene, PDR5, and causes pleiotropic drug resistance (PDR). Although a histone deacetylase, Rpd3, is also required for cycloheximide resistance in ρ0/- cells, it is currently unknown whether Rpd3 and its DNA binding partners, Ume6 and Ash1, are involved in the activation of PDR5 transcription and PDR in ρ0/- cells. This study investigated the roles of RPD3, UME6, and ASH1 in the activation of PDR5 transcription and PDR by retrograde signalling in ρ0 cells. RESULTS:ρ0 cells in the rpd3∆ and ume6∆ strains, with the exception of the ash1∆ strain, were sensitive to fluconazole and cycloheximide. The PDR5 mRNA levels in ρ0 cells of the rpd3∆ and ume6∆ strains were significantly reduced compared to the wild-type and ash1∆ strain. Transcriptional expression of PDR5 was reduced in cycloheximide-exposed and unexposed ρ0 cells of the ume6∆ strain; the transcriptional positive response of PDR5 to cycloheximide exposure was also impaired in this strain. CONCLUSIONS:RPD3 and UME6 are responsible for enhanced PDR5 mRNA levels and PDR by retrograde signalling in ρ0 cells of S. cerevisiae. |
| 巻・号 | 21(1) |
| ページ | 311 |
| 公開日 | 2021-11-9 |
| DOI | 10.1186/s12866-021-02373-1 |
| PII | 10.1186/s12866-021-02373-1 |
| PMID | 34753419 |
| PMC | PMC8576940 |
| MeSH | ATP-Binding Cassette Transporters / genetics* ATP-Binding Cassette Transporters / metabolism Antifungal Agents / pharmacology* Cycloheximide / pharmacology Fluconazole / pharmacology Gene Expression Regulation, Fungal* / drug effects Histone Deacetylases / genetics Histone Deacetylases / metabolism* Mitochondria / drug effects Mitochondria / genetics Mitochondria / metabolism Repressor Proteins / genetics Repressor Proteins / metabolism* Saccharomyces cerevisiae / drug effects Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins / genetics* Saccharomyces cerevisiae Proteins / metabolism* Transcriptional Activation / drug effects |
| IF | 2.989 |
| リソース情報 | |
| 酵母 | BY4848 |