論文 - 詳細
| RRC ID | 70674 |
|---|---|
| 著者 | Huang CX, Zhao Y, Mao J, Wang Z, Xu L, Cheng J, Guan NN, Song J. |
| タイトル | An injury-induced serotonergic neuron subpopulation contributes to axon regrowth and function restoration after spinal cord injury in zebrafish. |
| ジャーナル | Nat Commun |
| Abstract |
Spinal cord injury (SCI) interrupts long-projecting descending spinal neurons and disrupts the spinal central pattern generator (CPG) that controls locomotion. The intrinsic mechanisms underlying re-wiring of spinal neural circuits and recovery of locomotion after SCI are unclear. Zebrafish shows axonal regeneration and functional recovery after SCI making it a robust model to study mechanisms of regeneration. Here, we use a two-cut SCI model to investigate whether recovery of locomotion can occur independently of supraspinal connections. Using this injury model, we show that injury induces the localization of a specialized group of intraspinal serotonergic neurons (ISNs), with distinctive molecular and cellular properties, at the injury site. This subpopulation of ISNs have hyperactive terminal varicosities constantly releasing serotonin activating 5-HT1B receptors, resulting in axonal regrowth of spinal interneurons. Axon regrowth of excitatory interneurons is more pronounced compared to inhibitory interneurons. Knock-out of htr1b prevents axon regrowth of spinal excitatory interneurons, negatively affecting coordination of rostral-caudal body movements and restoration of locomotor function. On the other hand, treatment with 5-HT1B receptor agonizts promotes functional recovery following SCI. In summary, our data show an intraspinal mechanism where a subpopulation of ISNs stimulates axonal regrowth resulting in improved recovery of locomotor functions following SCI in zebrafish. |
| 巻・号 | 12(1) |
| ページ | 7093 |
| 公開日 | 2021-12-7 |
| DOI | 10.1038/s41467-021-27419-w |
| PII | 10.1038/s41467-021-27419-w |
| PMID | 34876587 |
| PMC | PMC8651775 |
| MeSH | Animals Axons / physiology* Electrophysiology Interneurons Locomotion Receptors, Serotonin / genetics Receptors, Serotonin / metabolism Recovery of Function* Serotonergic Neurons / pathology Serotonergic Neurons / physiology* Serotonin / metabolism Spinal Cord / physiopathology Spinal Cord Injuries* / genetics Spinal Cord Injuries* / metabolism Spinal Cord Injuries* / pathology Zebrafish |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(UAS:nfsB-mCherry)rw0144 Tg(vglut2a:Gal4) Tg(UAS:GFP) Tg(glyt2:GFP) |