RRC ID 70752
Author Chang X, Zhang F, Li H, Mo D, Shen J, Zhang J.
Title Characterization of a new mastermind allele identified from somatic mosaic screen.
Journal Cells Dev
Abstract The Notch signaling pathway is highly conserved and regulates various fundamental development events. Activation of Notch signaling relies on production of the Notch intracellular domain (NICD), which assembles a transcription factor complex to turn on down-stream targets expression. The mastermind (mam) gene encodes an essential co-activator that permits NICD activity in the cell nucleus. During a somatic mosaic screen in Drosophila, an uncharacterized gene l(2)S9998 is identified as a positive regulator of the Notch signaling pathway. Genetic analysis demonstrates that l(2)S9998 functions at the level of transcriptional activation of Notch targets in the signal receiving cells. Whole genome sequencing reveals that l(2)S9998 is a novel allele of the mam gene, which is further confirmed by complementation tests. Along with three molecularly defined transposon insertions isolated from the screen, four mutants of mam are shown to modulate Notch signaling during fly wing development. Our analysis provides additional genetic resources for understanding mam function and Notch signaling regulation.
Volume 165
Pages 203664
Published 2021-3-1
DOI 10.1016/j.cdev.2021.203664
PII S2667-2901(21)00011-5
PMID 33993981
MeSH Alleles* Animals Chromosome Mapping DNA Transposable Elements / genetics Drosophila Proteins / genetics* Drosophila melanogaster / genetics* Genetic Testing* Mutagenesis, Insertional / genetics Nuclear Proteins / genetics* Receptors, Notch / metabolism Signal Transduction Wings, Animal / embryology
Drosophila DGRC#111436 DGRC#111501 DGRC#111069 DGRC#114414