RRC ID 70755
Author Canales Coutiño B, Szamek E, Markus Z, Georgiou M.
Title Generation and live imaging of tumors with specific genotypes in the living fly pupa.
Journal STAR Protoc
Abstract This protocol describes the step-by-step generation of tumors with specific genotypes on the dorsal thorax epithelium of the fly. This in vivo system allows the imaging of tumor cell morphology and behavior in high spatial and temporal resolution. Phenotypes such as cell invasion, cell division, and tumor size can be quantified and compared to specific controls or to the neighboring wild-type tissue. Thus, this model allows the study of conserved genes that enhance or suppress epithelial tumor progression. For complete details on the use and execution of this protocol, please refer to Canales Coutiño et al. (2020).
Volume 2(3)
Pages 100672
Published 2021-9-17
DOI 10.1016/j.xpro.2021.100672
PII S2666-1667(21)00379-8
PMID 34355201
PMC PMC8319805
MeSH Animals Animals, Genetically Modified Crosses, Genetic Drosophila Proteins / genetics Drosophila melanogaster / genetics* Gene Expression Regulation, Neoplastic Genotype Green Fluorescent Proteins / genetics Microscopy, Confocal / methods* Neoplasms, Experimental / genetics* Neoplasms, Experimental / pathology* Pupa* / genetics Tumor Suppressor Proteins / genetics