RRC ID 70756
Author Resnik-Docampo M, Cunningham KM, Ruvalcaba SM, Choi C, Sauer V, Jones DL.
Title Neuroglian regulates Drosophila intestinal stem cell proliferation through enhanced signaling via the epidermal growth factor receptor.
Journal Stem Cell Reports
Abstract The Drosophila intestine is an excellent system for elucidating mechanisms regulating stem cell behavior. Here we show that the septate junction (SJ) protein Neuroglian (Nrg) is expressed in intestinal stem cells (ISCs) and enteroblasts (EBs) within the fly intestine. SJs are not present between ISCs and EBs, suggesting Nrg plays a different role in this tissue. We reveal that Nrg is required for ISC proliferation in young flies, and depletion of Nrg from ISCs and EBs suppresses increased ISC proliferation in aged flies. Conversely, overexpression of Nrg in ISC and EBs promotes ISC proliferation, leading to an increase in cells expressing ISC/EB markers; in addition, we observe an increase in epidermal growth factor receptor (Egfr) activation. Genetic epistasis experiments reveal that Nrg acts upstream of Egfr to regulate ISC proliferation. As Nrg function is highly conserved in mammalian systems, our work characterizing the role of Nrg in the intestine has implications for the treatment of intestinal disorders that arise due to altered ISC behavior.
Volume 16(6)
Pages 1584-1597
Published 2021-6-8
DOI 10.1016/j.stemcr.2021.04.006
PII S2213-6711(21)00201-0
PMID 33961791
PMC PMC8190597
MeSH Aging / metabolism Animals Cell Adhesion Molecules, Neuronal / genetics Cell Adhesion Molecules, Neuronal / metabolism* Cell Proliferation Drosophila / metabolism* Drosophila Proteins / genetics Drosophila Proteins / metabolism* ErbB Receptors / metabolism* Female Gene Expression Regulation, Developmental Intestines / cytology Intestines / metabolism* Signal Transduction Stem Cells / metabolism*
IF 6.032
Drosophila DGRC#111923