RRC ID 70770
Author di Pietro F, Herszterg S, Huang A, Bosveld F, Alexandre C, Sancéré L, Pelletier S, Joudat A, Kapoor V, Vincent JP, Bellaïche Y.
Title Rapid and robust optogenetic control of gene expression in Drosophila.
Journal Dev Cell
Abstract Deciphering gene function requires the ability to control gene expression in space and time. Binary systems such as the Gal4/UAS provide a powerful means to modulate gene expression and to induce loss or gain of function. This is best exemplified in Drosophila, where the Gal4/UAS system has been critical to discover conserved mechanisms in development, physiology, neurobiology, and metabolism, to cite a few. Here we describe a transgenic light-inducible Gal4/UAS system (ShineGal4/UAS) based on Magnet photoswitches. We show that it allows efficient, rapid, and robust activation of UAS-driven transgenes in different tissues and at various developmental stages in Drosophila. Furthermore, we illustrate how ShineGal4 enables the generation of gain and loss-of-function phenotypes at animal, organ, and cellular levels. Thanks to the large repertoire of UAS-driven transgenes, ShineGal4 enriches the Drosophila genetic toolkit by allowing in vivo control of gene expression with high temporal and spatial resolutions.
Volume 56(24)
Pages 3393-3404.e7
Published 2021-12-20
DOI 10.1016/j.devcel.2021.11.016
PII S1534-5807(21)00940-0
PMID 34879263
PMC PMC8693864
MeSH Animals Body Patterning / genetics Body Patterning / radiation effects Drosophila melanogaster / genetics* Drosophila melanogaster / radiation effects Gene Expression Regulation, Developmental* / radiation effects Light Optogenetics* Organ Specificity / genetics Organ Specificity / radiation effects Pupa / genetics Pupa / radiation effects Time Factors
IF 10.092