RRC ID 70781
Author Wada Y, Ohsawa S, Igaki T.
Title Yorkie ensures robust tissue growth in Drosophila ribosomal protein mutants.
Journal Development
Abstract Heterozygosity of ribosomal protein genes causes a variety of developmental abnormalities in humans, which are collectively known as ribosomopathies, yet the underlying mechanisms remain elusive. Here, we analyzed Drosophila Minute (M)/+ mutants, a group of mutants heterozygous for ribosomal protein genes that exhibit a characteristic thin-bristle phenotype. We found that, although M/+ flies develop essentially normal wings, simultaneous deletion of one copy of the Hippo pathway effector yki resulted in severe wing growth defects. These defects were caused by JNK-mediated cell death in the wing pouch via Eiger/TNF signaling. The JNK activation in M/+, yki/+ wing discs required the caspase Dronc, which is normally blocked by DIAP1. Notably, heterozygosity of yki reduced DIAP1 expression in the wing pouch, leading to elevation of Dronc activity. Dronc and JNK formed a positive-feedback loop that amplifies Dronc activation, leading to apoptosis. Our observations suggest a mechanism of robust tissue growth whereby tissues with reduced ribosomal protein prevent ectopic apoptosis via Yki activity.
Volume 148(14)
Published 2021-7-15
DOI 10.1242/dev.198705
PII 271054
PMID 34313318
MeSH Animals Apoptosis Cell Death Down-Regulation Drosophila / genetics Drosophila / metabolism* Drosophila Proteins / genetics* Drosophila Proteins / metabolism* Female Gene Expression Regulation, Developmental Inhibitor of Apoptosis Proteins / metabolism Nuclear Proteins / genetics Ribosomal Proteins / genetics* Ribosomal Proteins / metabolism* Signal Transduction Trans-Activators / genetics Wings, Animal / anatomy & histology Wings, Animal / metabolism YAP-Signaling Proteins
IF 5.611
Resource
Drosophila DGRC#114620 DGRC#101668 DGRC#107282 12284R-2