RRC ID 70853
Author Wei JY, Chu SY, Huang YC, Chung PC, Yu HH.
Title Drosophila septin interacting protein 1 regulates neurogenesis in the early developing larval brain.
Journal Sci Rep
Abstract Neurogenesis in the Drosophila central brain progresses dynamically in order to generate appropriate numbers of neurons during different stages of development. Thus, a central challenge in neurobiology is to reveal the molecular and genetic mechanisms of neurogenesis timing. Here, we found that neurogenesis is significantly impaired when a novel mutation, Nuwa, is induced at early but not late larval stages. Intriguingly, when the Nuwa mutation is induced in neuroblasts of olfactory projection neurons (PNs) at the embryonic stage, embryonic-born PNs are generated, but larval-born PNs of the same origin fail to be produced. Through molecular characterization and transgenic rescue experiments, we determined that Nuwa is a loss-of-function mutation in Drosophila septin interacting protein 1 (sip1). Furthermore, we found that SIP1 expression is enriched in neuroblasts, and RNAi knockdown of sip1 using a neuroblast driver results in formation of small and aberrant brains. Finally, full-length SIP1 protein and truncated SIP1 proteins lacking either the N- or C-terminus display different subcellular localization patterns, and only full-length SIP1 can rescue the Nuwa-associated neurogenesis defect. Taken together, these results suggest that SIP1 acts as a crucial factor for specific neurogenesis programs in the early developing larval brain.
Volume 12(1)
Pages 292
Published 2022-1-7
DOI 10.1038/s41598-021-04474-3
PII 10.1038/s41598-021-04474-3
PMID 34997175
PMC PMC8742078
MeSH Animals Animals, Genetically Modified Brain / embryology Brain / metabolism* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / embryology Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Gene Expression Regulation, Developmental Larva / genetics Larva / metabolism Loss of Function Mutation Neurogenesis* Neurons / metabolism* Signal Transduction
IF 3.998
Drosophila DGRC#111477