RRC ID |
7089
|
著者 |
Steinsbu BO, Thorseth IH, Nakagawa S, Inagaki F, Lever MA, Engelen B, Øvreås L, Pedersen RB.
|
タイトル |
Archaeoglobus sulfaticallidus sp. nov., a thermophilic and facultatively lithoautotrophic sulfate-reducer isolated from black rust exposed to hot ridge flank crustal fluids.
|
ジャーナル |
Int J Syst Evol Microbiol
|
Abstract |
A novel thermophilic and lithoautotrophic sulfate-reducing archaeon was isolated from black rust formed on the steel surface of a borehole observatory (CORK 1026B) retrieved during IODP Expedition 301 on the eastern flank of Juan de Fuca Ridge, eastern Pacific Ocean. Cells of the strain were lobe-shaped or triangular. The optimum temperature, pH and NaCl concentration for growth were 75°C, pH 7 and 2 % (w/v), respectively. The isolate was strictly anaerobic, growing lithoautotrophically on H(2) and CO(2) using sulfate, sulfite or thiosulfate as electron acceptors. Lactate and pyruvate could serve as alternative energy and carbon sources. The G+C content of the genomic DNA was 42 mol%. Phylogenetic analyses of the 16S rRNA gene indicated that the isolate was closely related to members of the family Archaeoglobaceae, with sequence similarities of 90.3-94.4 %. Physiological and molecular properties showed that the isolate represents a novel species of the genus Archaeoglobus. The name Archaeoglobus sulfaticallidus sp. nov. is proposed; the type strain is PM70-1(T) (=DSM 19444(T)=JCM 14716(T)).
|
巻・号 |
60(Pt 12)
|
ページ |
2745-2752
|
公開日 |
2010-12-1
|
DOI |
10.1099/ijs.0.016105-0
|
PMID |
20061497
|
MeSH |
Archaeoglobus / classification*
Archaeoglobus / genetics
Archaeoglobus / isolation & purification
Bacterial Typing Techniques
Base Composition
DNA, Archaeal / genetics
Hot Temperature
Molecular Sequence Data
Oxidation-Reduction
Pacific Ocean
Phylogeny*
RNA, Ribosomal, 16S / genetics
Seawater / microbiology*
Sequence Analysis, DNA
Sulfates / metabolism
|
IF |
2.415
|
引用数 |
29
|
WOS 分野
|
MICROBIOLOGY
|
リソース情報 |
一般微生物 |
JCM 14716 |