| RRC ID |
70957
|
| Author |
Kanchanasin P, Yuki M, Kudo T, Ohkuma M, Phongsopitanun W, Tanasupawat S.
|
| Title |
Nocardia terrae sp. nov., an actinomycete isolated from soil in Thailand.
|
| Journal |
Arch Microbiol
|
| Abstract |
A novel actinomycete strain ET3-3T isolated from soil collected from Chachoengsao province, Thailand was taxonomic evaluated using a polyphasic approach. Chemotaxonomic analysis indicated that meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. Mycolic acids were present. Ribose, arabinose, and galactose were detected in its whole-cell hydrolysates. The strain comprised C16:0 and TBSA 10-methyl C18:0 as the main fatty acids and MK-8(H4ω-cycl) as the predominant menaquinone. Major polar lipids were phosphatidylinositol, phosphatidylinositol mannoside, phosphatidylethanolamine, and diphosphatidylglycerol. The results of 16S rRNA gene sequence analysis showed that strain ET3-3T was closely related to Nocardia seriolae JCM 30082T (99.2%), Nocardia yunnanensis JCM 30082T (98.4%), and Nocardia concava JCM 12351T (98.2%). The draft genome of ET3-3T was 9.31 Mb with 8826 coding sequences with an average G + C content of 68.0%. The comparison of the draft genome of strain ET3-3T and N. seriolae NBRC 15557T showed the ANIb, ANIm and the digital DNA-DNA hybridization values of 86.3%, 88.5% and 32.9%, respectively. The results of the taxonomic analysis suggested that strain ET3-3T represented a novel species of the genus Nocardia for which the name Nocardia terrae sp. nov. is proposed. The type strain is ET3-3T (= JCM 33776T = TISTR 2837T).
|
| Volume |
203(3)
|
| Pages |
1071-1077
|
| Published |
2021-4-1
|
| DOI |
10.1007/s00203-020-02107-3
|
| PII |
10.1007/s00203-020-02107-3
|
| PMID |
33146799
|
| MeSH |
Base Composition
Fatty Acids / chemistry
Nocardia / classification*
Nocardia / genetics
Nocardia / isolation & purification
Phospholipids / chemistry
RNA, Ribosomal, 16S / genetics
Soil Microbiology*
Species Specificity
Thailand
|
| Resource |
| General Microbes |
JCM33776
JCM30082
JCM12351
JCM3360 |
