| RRC ID |
7097
|
| 著者 |
Nagaoka S, Minegishi H, Echigo A, Usami R.
|
| タイトル |
Halostagnicola kamekurae sp. nov., an extremely halophilic archaeon from solar salt.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
A novel extremely halophilic archaeon, strain 194-10(T), was isolated from a solar salt sample imported into Japan from the Philippines. Strain 194-10(T) was pleomorphic, neutrophilic and mesophilic and required at least 10 % (w/v) NaCl but no MgSO(4) . 7H(2)O for growth; it exhibited optimal growth at 15 % (w/v) NaCl and 60 mM MgSO(4) . 7H(2)O. Strain 194-10(T) grew at 20-45°C (optimum, 30°C) and pH 6.0-9.0 (optimum, pH 6.5-7.0). The G+C content of its DNA was 59.8 mol%. 16S rRNA gene sequence analysis revealed closest proximity to Halostagnicola larsenii XH-48(T) (98.5 % similarity), the sole representative of the genus Halostagnicola. Polar lipid analysis revealed that strain 194-10(T) contained phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester (the latter derived from both C(20)C(20) and C(20)C(25) archaeol) and several unidentified glycolipids. The results of DNA-DNA hybridization (20.7 % relatedness between Hst. larsenii JCM 13463(T) and strain 194-10(T)) and physiological and biochemical characteristics allowed differentiation of strain 194-10(T) from Hst. larsenii XH-48(T). Therefore, strain 194-10(T) represents a novel species of the genus Halostagnicola, for which the name Halostagnicola kamekurae sp. nov. is proposed, with the type strain 194-10(T) (=DSM 22427(T) =JCM 16110(T) =CECT 7536(T)).
|
| 巻・号 |
60(Pt 12)
|
| ページ |
2828-2831
|
| 公開日 |
2010-12-1
|
| DOI |
10.1099/ijs.0.014449-0
|
| PMID |
20081018
|
| MeSH |
Base Composition
DNA, Archaeal / genetics
Halobacteriaceae / classification*
Halobacteriaceae / genetics
Halobacteriaceae / isolation & purification
Lipids / chemistry
Molecular Sequence Data
Nucleic Acid Hybridization
Philippines
Phylogeny*
RNA, Ribosomal, 16S / genetics
Salts
Sequence Analysis, DNA
|
| IF |
2.415
|
| 引用数 |
10
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 16110
JCM 13463 |
