| RRC ID |
71020
|
| 著者 |
Yokoi KJ, Tsutsui S, Arakawa GY, Takaba M, Fujii K, Kaneko S.
|
| タイトル |
Molecular and biochemical characteristics of inulosucrase InuBK from Alkalihalobacillus krulwichiae JCM 11691.
|
| ジャーナル |
Biosci Biotechnol Biochem
|
| Abstract |
Information about the inulosucrase of nonlactic acid bacteria is scarce. We found a gene encoding inulosucrase (inuBK) in the genome of the Gram-positive bacterium Alkalihalobacillus krulwichiae JCM 11691. The inuBK open reading frame encoded a protein comprising 456 amino acids. We expressed His-tagged InuBK in culture medium using a Brevibacillus system. The optimal pH and temperature of purified InuBK were 7.0-9.0 and 50-55 °C, respectively. The findings of high-performance anion-exchange chromatography, nuclear magnetic resonance spectroscopy, and high-performance size-exclusion chromatography with multiangle laser light scattering showed that the polysaccharide produced by InuBK was an inulin with a molecular weight of 3806, a polydispersity index (PI) of 1.047, and fructosyl chain lengths with 3-27 degrees of polymerization. The size of InuBK was smaller than commercial inulins, and the PI of the inulin that it produced was lower.
|
| 巻・号 |
85(8)
|
| ページ |
1830-1838
|
| 公開日 |
2021-7-23
|
| DOI |
10.1093/bbb/zbab094
|
| PII |
6280973
|
| PMID |
34021568
|
| MeSH |
Bacillaceae / enzymology*
Bacillaceae / genetics
Chromatography, High Pressure Liquid / methods
Cloning, Molecular
Culture Media
Genes, Bacterial
Hexosyltransferases / genetics
Hexosyltransferases / isolation & purification
Hexosyltransferases / metabolism*
Inulin / biosynthesis
Magnetic Resonance Spectroscopy / methods
Molecular Weight
Phylogeny
Temperature
|
| リソース情報 |
| 一般微生物 |
JCM11691 |
