Reference - Detail
| RRC ID | 71066 |
|---|---|
| Author | Yoshida M, Sano S, Chien JY, Fukano H, Suzuki M, Asakura T, Morimoto K, Murase Y, Miyamoto S, Kurashima A, Hasegawa N, Hsueh PR, Mitarai S, Ato M, Hoshino Y. |
| Title | A novel DNA chromatography method to discriminate Mycobacterium abscessus subspecies and macrolide susceptibility. |
| Journal | EBioMedicine |
| Abstract |
BACKGROUND:The clinical impact of infection with Mycobacterium (M.) abscessus complex (MABC), a group of emerging non-tuberculosis mycobacteria (NTM), is increasing. M. abscessus subsp. abscessus/bolletii frequently shows natural resistance to macrolide antibiotics, whereas M. abscessus subsp. massiliense is generally susceptible. Therefore, rapid and accurate discrimination of macrolide-susceptible MABC subgroups is required for effective clinical decisions about macrolide treatments for MABC infection. We aimed to develop a simple and rapid diagnostic that can identify MABC isolates showing macrolide susceptibility. METHODS:Whole genome sequencing (WGS) was performed for 148 clinical or environmental MABC isolates from Japan to identify genetic markers that can discriminate three MABC subspecies and the macrolide-susceptible erm(41) T28C sequevar. Using the identified genetic markers, we established PCR based- or DNA chromatography-based assays. Validation testing was performed using MABC isolates from Taiwan. FINDING:We identified unique sequence regions that could be used to differentiate the three subspecies. Our WGS-based phylogenetic analysis indicated that M. abscessus carrying the macrolide-susceptible erm(41) T28C sequevar were tightly clustered, and identified 11 genes that were significantly associated with the lineage for use as genetic markers. To detect these genetic markers and the erm(41) locus, we developed a DNA chromatography method that identified three subspecies, the erm(41) T28C sequevar and intact erm(41) for MABC in a single assay within one hour. The agreement rate between the DNA chromatography-based and WGS-based identification was 99·7%. INTERPRETATION:We developed a novel, rapid and simple DNA chromatography method for identification of MABC macrolide susceptibility with high accuracy. FUNDING:AMED, JSPS KAKENHI. |
| Volume | 64 |
| Pages | 103187 |
| Published | 2021-2-1 |
| DOI | 10.1016/j.ebiom.2020.103187 |
| PII | S2352-3964(20)30563-6 |
| PMID | 33446475 |
| PMC | PMC7910664 |
| MeSH | Anti-Bacterial Agents / pharmacology* Chromatography / methods* DNA / analysis* Drug Resistance, Bacterial / drug effects Genes, Bacterial Humans Macrolides / pharmacology* Microbial Sensitivity Tests Mycobacterium abscessus / classification Mycobacterium abscessus / drug effects* Mycobacterium abscessus / genetics* Phylogeny Polymerase Chain Reaction |
| Resource | |
| General Microbes | JCM13569 JCM15300 JCM15297 JCM6388 JCM15299 JCM6387 JCM6382 JCM15656 JCM6379 JCM13390 JCM15467 JCM6386 JCM14744 JCM15661 |