| RRC ID |
71236
|
| Author |
Phongsopitanun W, Kanchanasin P, Sripreechasak P, Rueangsawang K, Athipornchai A, Supong K, Pittayakhajonwut P, Tanasupawat S.
|
| Title |
Potential antibiotic production of Streptomyces justiciae sp. nov., isolated from the root of Justicia subcoriacea.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
Endophytic actinobacterial strain 3R004T was isolated from a root of Justicia subcoriacea collected in Thailand. In this report, the taxonomic position of this strain is described using a polyphasic approach. Based on the morphological characteristics and chemical composition of its cells, strain 3R004T was identified as a member of the genus Streptomyces. It produced a long chain of cylindrical spores on aerial mycelia. ll-Diaminopimelic acid was detected in the cell wall peptidoglycan. The menaquinones were MK-9(H4), MK-9(H6) and MK-9(H8). C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and iso-C15 : 0 were detected as the major cellular fatty acids. Polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and one unidentified lipid. Strain 3R004T showed the highest 16S rRNA gene similarity of 99.45 % to Streptomyces cyaneochromogenes MK-45T. The phylogenomic results indicated that strain 3R004T was close to Streptomyces aquilus GGCR-6T and Streptomyces antibioticus DSM 40234T. The DNA-DNA hybridization and average nucleotide identity values among strain 3R004T and closely related Streptomyces species were 35.5-63.1 % and 82.7-94.3 %, respectively. The type strain produced actinomycin D antibiotic as the major secondary metabolite. The maximum productivity of the actinomycin D (378 mg l-1) was observed when the strain was grown in 301 broth at 30 °C, 180 r.p.m. for 12 days. On the basis of phenotypic and genotypic evidence, strain 3R004T represents a novel species of the genus Streptomyces, for which the name Streptomyces justiciae is proposed. The type strain is 3R004T (=LMG 32138T=TBRC 13128T=NBRC 115065T).
|
| Volume |
71(9)
|
| Published |
2021-9-1
|
| DOI |
10.1099/ijsem.0.005017
|
| PMID |
34550061
|
| MeSH |
Anti-Bacterial Agents
Bacterial Typing Techniques
Base Composition
DNA, Bacterial / genetics
Fatty Acids / chemistry
Justicia*
Phospholipids
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Streptomyces* / genetics
Vitamin K 2
|
| Resource |
| General Microbes |
JCM4620
JCM33584 |
