論文 - 詳細
| RRC ID | 71378 |
|---|---|
| 著者 | Ohkubo T, Matsumoto Y, Cho O, Ogasawara Y, Sugita T. |
| タイトル | Delftia acidovorans secretes substances that inhibit the growth of Staphylococcus epidermidis through TCA cycle-triggered ROS production. |
| ジャーナル | PLoS One |
| Abstract |
The proportion of Staphylococcus aureus in the skin microbiome is associated with the severity of inflammation in the skin disease atopic dermatitis. Staphylococcus epidermidis, a commensal skin bacterium, inhibits the growth of S. aureus in the skin. Therefore, the balance between S. epidermidis and S. aureus in the skin microbiome is important for maintaining healthy skin. In the present study, we demonstrated that the heat-treated culture supernatant of Delftia acidovorans, a member of the skin microbiome, inhibits the growth of S. epidermidis, but not that of S. aureus. Comprehensive gene expression analysis by RNA sequencing revealed that culture supernatant of D. acidovorans increased the expression of genes related to glycolysis and the tricarboxylic acid cycle (TCA) cycle in S. epidermidis. Malonate, an inhibitor of succinate dehydrogenase in the TCA cycle, suppressed the inhibitory effect of the heat-treated culture supernatant of D. acidovorans on the growth of S. epidermidis. Reactive oxygen species production in S. epidermidis was induced by the heat-treated culture supernatant of D. acidovorans and suppressed by malonate. Further, the inhibitory effect of the heat-treated culture supernatant of D. acidovorans on the growth of S. epidermidis was suppressed by N-acetyl-L-cysteine, a free radical scavenger. These findings suggest that heat-resistant substances secreted by D. acidovorans inhibit the growth of S. epidermidis by inducing the production of reactive oxygen species via the TCA cycle. |
| 巻・号 | 16(7) |
| ページ | e0253618 |
| 公開日 | 2021-1-1 |
| DOI | 10.1371/journal.pone.0253618 |
| PII | PONE-D-21-01227 |
| PMID | 34214099 |
| PMC | PMC8253425 |
| MeSH | Bacterial Proteins / genetics Bacterial Proteins / immunology Bacterial Proteins / metabolism Citric Acid Cycle / immunology Delftia acidovorans / genetics Delftia acidovorans / immunology* Delftia acidovorans / metabolism Dermatitis, Atopic / immunology* Dermatitis, Atopic / microbiology Dermatitis, Atopic / pathology Gene Expression Regulation, Bacterial / immunology Humans Microbiota / immunology RNA-Seq Reactive Oxygen Species / metabolism Skin / immunology Skin / microbiology* Skin / pathology Staphylococcal Infections / immunology* Staphylococcal Infections / microbiology Staphylococcal Infections / pathology Staphylococcus aureus / immunology Staphylococcus aureus / isolation & purification Staphylococcus epidermidis / immunology Staphylococcus epidermidis / isolation & purification* |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM6218 |