論文 - 詳細
| RRC ID | 71386 |
|---|---|
| 著者 | Omata K, Hibi N, Nakano S, Komoto S, Sato K, Nunokawa K, Amano S, Ueda K, Takano H. |
| タイトル | Distribution and genome structures of temperate phages in acetic acid bacteria. |
| ジャーナル | Sci Rep |
| Abstract |
Acetic acid bacteria (AAB) are industrial microorganisms used for vinegar fermentation. Herein, we investigated the distribution and genome structures of mitomycin C-inducible temperate phages in AAB. Transmission electron microscopy analysis revealed phage-like particles in 15 out of a total 177 acetic acid bacterial strains, all of which showed morphology similar to myoviridae-type phage. The complete genome sequences of the six phages derived from three strains each of Acetobacter and Komagataeibacter strains were determined, harboring a genome size ranging from 34,100 to 53,798 bp. A phage AP1 from A. pasteurianus NBRC 109446 was predicted as an active phage based on the genomic information, and actually had the ability to infect its phiAP1-cured strain. The attachment sites for phiAP1 were located in the 3'-end region of the tRNAser gene. We also developed a chromosome-integrative vector, p2096int, based on the integrase function of phiAP1, and it was successfully integrated into the attachment site of the phiAP1-cured strain, which may be used as a valuable tool for the genetic engineering. Overall, this study showed the distribution of mitomycin C-inducible temperate phages in AAB, and identified the active temperate phage o f A. pasteurianus. |
| 巻・号 | 11(1) |
| ページ | 21567 |
| 公開日 | 2021-11-3 |
| DOI | 10.1038/s41598-021-00998-w |
| PII | 10.1038/s41598-021-00998-w |
| PMID | 34732777 |
| PMC | PMC8566455 |
| MeSH | Acetic Acid / chemistry* Acetobacter Acetobacteraceae Ampicillin Bacteria / genetics* Bacteriophages Cluster Analysis Computational Biology Fermentation Genetic Engineering Genome, Bacterial Genomics Microscopy, Electron, Transmission Mitomycin / chemistry* Myoviridae Open Reading Frames Phylogeny Plasmids / metabolism Saccharomycetales Temperature |
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オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM6891 |