| RRC ID |
71386
|
| 著者 |
Omata K, Hibi N, Nakano S, Komoto S, Sato K, Nunokawa K, Amano S, Ueda K, Takano H.
|
| タイトル |
Distribution and genome structures of temperate phages in acetic acid bacteria.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Acetic acid bacteria (AAB) are industrial microorganisms used for vinegar fermentation. Herein, we investigated the distribution and genome structures of mitomycin C-inducible temperate phages in AAB. Transmission electron microscopy analysis revealed phage-like particles in 15 out of a total 177 acetic acid bacterial strains, all of which showed morphology similar to myoviridae-type phage. The complete genome sequences of the six phages derived from three strains each of Acetobacter and Komagataeibacter strains were determined, harboring a genome size ranging from 34,100 to 53,798 bp. A phage AP1 from A. pasteurianus NBRC 109446 was predicted as an active phage based on the genomic information, and actually had the ability to infect its phiAP1-cured strain. The attachment sites for phiAP1 were located in the 3'-end region of the tRNAser gene. We also developed a chromosome-integrative vector, p2096int, based on the integrase function of phiAP1, and it was successfully integrated into the attachment site of the phiAP1-cured strain, which may be used as a valuable tool for the genetic engineering. Overall, this study showed the distribution of mitomycin C-inducible temperate phages in AAB, and identified the active temperate phage o f A. pasteurianus.
|
| 巻・号 |
11(1)
|
| ページ |
21567
|
| 公開日 |
2021-11-3
|
| DOI |
10.1038/s41598-021-00998-w
|
| PII |
10.1038/s41598-021-00998-w
|
| PMID |
34732777
|
| PMC |
PMC8566455
|
| MeSH |
Acetic Acid / chemistry*
Acetobacter
Acetobacteraceae
Ampicillin
Bacteria / genetics*
Bacteriophages
Cluster Analysis
Computational Biology
Fermentation
Genetic Engineering
Genome, Bacterial
Genomics
Microscopy, Electron, Transmission
Mitomycin / chemistry*
Myoviridae
Open Reading Frames
Phylogeny
Plasmids / metabolism
Saccharomycetales
Temperature
|
| リソース情報 |
| 一般微生物 |
JCM6891 |
