RRC ID 7140
Author Sasaki T, Tsubakishita S, Tanaka Y, Sakusabe A, Ohtsuka M, Hirotaki S, Kawakami T, Fukata T, Hiramatsu K.
Title Multiplex-PCR method for species identification of coagulase-positive staphylococci.
Journal J. Clin. Microbiol.
Abstract In veterinary medicine, coagulase-positive staphylococci (CoPS) other than Staphylococcus aureus have frequently been misidentified as being S. aureus strains, as they have several phenotypic traits in common. There has been no reliable method to distinguish among CoPS species in veterinary clinical laboratories. In the present study, we sequenced the thermonuclease (nuc) genes of staphylococcal species and devised a multiplex-PCR (M-PCR) method for species identification of CoPS by targeting the nuc gene locus. To evaluate sensitivity and specificity, we used this M-PCR method on 374 staphylococcal strains that had been previously identified to the species level by an hsp60 sequencing approach. We could successfully distinguish between S. aureus, S. hyicus, S. schleiferi, S. intermedius, S. pseudintermedius, and S. delphini groups A and B. The present method was both sensitive (99.8%) and specific (100%). Our M-PCR assay will allow the routine species identification of CoPS isolates from various animal species for clinical veterinary diagnosis.
Volume 48(3)
Pages 765-9
Published 2010-3
DOI 10.1128/JCM.01232-09
PII JCM.01232-09
PMID 20053855
PMC PMC2832457
MeSH Animals Bacteriological Techniques / methods* DNA Primers / genetics DNA, Bacterial / chemistry DNA, Bacterial / genetics Micrococcal Nuclease / genetics Molecular Sequence Data Polymerase Chain Reaction / methods* Polymorphism, Genetic Sensitivity and Specificity Staphylococcal Infections / diagnosis Staphylococcal Infections / microbiology Staphylococcal Infections / veterinary* Staphylococcus / classification Staphylococcus / genetics* Staphylococcus / isolation & purification* Veterinary Medicine / methods*
IF 4.054
Times Cited 130
General Microbes JCM 7470 JCM 2423 JCM 7469