論文 - 詳細
| RRC ID | 7140 |
|---|---|
| 著者 | Sasaki T, Tsubakishita S, Tanaka Y, Sakusabe A, Ohtsuka M, Hirotaki S, Kawakami T, Fukata T, Hiramatsu K. |
| タイトル | Multiplex-PCR method for species identification of coagulase-positive staphylococci. |
| ジャーナル | J Clin Microbiol |
| Abstract |
In veterinary medicine, coagulase-positive staphylococci (CoPS) other than Staphylococcus aureus have frequently been misidentified as being S. aureus strains, as they have several phenotypic traits in common. There has been no reliable method to distinguish among CoPS species in veterinary clinical laboratories. In the present study, we sequenced the thermonuclease (nuc) genes of staphylococcal species and devised a multiplex-PCR (M-PCR) method for species identification of CoPS by targeting the nuc gene locus. To evaluate sensitivity and specificity, we used this M-PCR method on 374 staphylococcal strains that had been previously identified to the species level by an hsp60 sequencing approach. We could successfully distinguish between S. aureus, S. hyicus, S. schleiferi, S. intermedius, S. pseudintermedius, and S. delphini groups A and B. The present method was both sensitive (99.8%) and specific (100%). Our M-PCR assay will allow the routine species identification of CoPS isolates from various animal species for clinical veterinary diagnosis. |
| 巻・号 | 48(3) |
| ページ | 765-9 |
| 公開日 | 2010-3-1 |
| DOI | 10.1128/JCM.01232-09 |
| PII | JCM.01232-09 |
| PMID | 20053855 |
| PMC | PMC2832457 |
| MeSH | Animals Bacteriological Techniques / methods* DNA Primers / genetics DNA, Bacterial / chemistry DNA, Bacterial / genetics Micrococcal Nuclease / genetics Molecular Sequence Data Polymerase Chain Reaction / methods* Polymorphism, Genetic Sensitivity and Specificity Staphylococcal Infections / diagnosis Staphylococcal Infections / microbiology Staphylococcal Infections / veterinary* Staphylococcus / classification Staphylococcus / genetics* Staphylococcus / isolation & purification* Veterinary Medicine / methods* |
| IF | 5.897 |
| 引用数 | 211 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Policy sources |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 7470 JCM 2423 JCM 7469 |