論文 - 詳細
| RRC ID | 7141 |
|---|---|
| 著者 | Kaji R, Kiyoshima-Shibata J, Nagaoka M, Nanno M, Shida K. |
| タイトル | Bacterial teichoic acids reverse predominant IL-12 production induced by certain lactobacillus strains into predominant IL-10 production via TLR2-dependent ERK activation in macrophages. |
| ジャーナル | J Immunol |
| Abstract |
The cytokine response of macrophages to probiotic lactobacilli varies between strains, and the balance of IL-10/IL-12 production is crucial for determination of the direction of the immune response. To clarify the mechanism whereby Lactobacillus strains differentially induce production of IL-10 and IL-12, we examined the potential relationship between cytokine production and MAPK activation. In mouse peritoneal macrophages, Lactobacillus plantarum potently induced IL-10 but weakly induced IL-12 production, whereas L. casei potently induced IL-12 but weakly induced IL-10 production. Kinetic analysis of the activation of ERK, p38, and JNK showed that L. plantarum induced a more rapid and intense activation of MAPKs, especially of ERK, than L. casei. A selective blockade of ERK activation induced by L. plantarum resulted in a decrease in IL-10 production and a simultaneous increase in IL-12 production. Interestingly, when macrophages were stimulated with a combination of L. plantarum and L. casei, IL-10 production was induced synergistically. We identified cell wall teichoic acid and lipoteichoic acid as key factors for triggering the synergistic induction of IL-10 production, although these teichoic acids alone only weakly induced IL-10 production. The effect of these teichoic acids on IL-10 production was mediated by TLR2-dependent ERK activation. Our data demonstrate that activation of the ERK pathway is critical for determination of the balance of the IL-10/IL-12 response of macrophages to lactobacilli and that predominant IL-12 production induced by certain lactobacilli such as L. casei can be converted into predominant IL-10 production when stimulated in the presence of teichoic acids. |
| 巻・号 | 184(7) |
| ページ | 3505-13 |
| 公開日 | 2010-4-1 |
| DOI | 10.4049/jimmunol.0901569 |
| PII | jimmunol.0901569 |
| PMID | 20190136 |
| MeSH | Animals Blotting, Western Cell Separation Enzyme Activation / immunology Extracellular Signal-Regulated MAP Kinases / immunology* Female Flow Cytometry Interleukin-10 / biosynthesis* Interleukin-10 / immunology Interleukin-12 / biosynthesis* Interleukin-12 / immunology Lactobacillus / immunology* Macrophages, Peritoneal / enzymology Macrophages, Peritoneal / immunology* Mice Mice, Inbred BALB C RNA, Messenger / analysis Signal Transduction / immunology Teichoic Acids / immunology* Toll-Like Receptor 2 / immunology |
| IF | 4.886 |
| 引用数 | 96 |
| WOS 分野 | IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 一般微生物 | JCM 2012 |