RRC ID 7143
著者 Sakamoto M, Ohkuma M.
タイトル Usefulness of the hsp60 gene for the identification and classification of Gram-negative anaerobic rods.
ジャーナル J Med Microbiol
Abstract The hsp60 gene sequences were determined for 121 strains of Gram-negative anaerobic rods, including the genera Bacteroides, Barnesiella, Butyricimonas, Odoribacter, Parabacteroides, Paraprevotella, Porphyromonas, Prevotella and Tannerella. The mean pairwise hsp60 gene sequence similarity (73.8-97.1 %) between species in each genus, except for the genus Tannerella that comprises one species, was significantly less than that of the 16S rRNA gene sequence (88.3-96.3 %). Only pairwise hsp60 gene sequence similarity (97.1 %) of the genus Paraprevotella was higher than that of the 16S rRNA gene sequence (93.8 %). Each genus formed a distinct clade in the phylogenetic analysis of the hsp60 gene sequence as well as the 16S rRNA gene sequence. The phylogenetic analysis indicated a higher evolutionary rate for the hsp60 gene sequence than the 16S rRNA gene sequence, especially in the genera Porphyromonas and Prevotella. This study suggests that the hsp60 gene is a useful alternative phylogenetic marker for the identification and classification of a broad range of Gram-negative anaerobic rods.
巻・号 59(Pt 11)
ページ 1293-1302
公開日 2010-11-1
DOI 10.1099/jmm.0.020420-0
PMID 20671088
MeSH Bacterial Proteins / genetics* Chaperonin 60 / genetics* Cluster Analysis DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / genetics Evolution, Molecular Genotype Gram-Negative Aerobic Rods and Cocci / classification* Gram-Negative Aerobic Rods and Cocci / genetics* Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sequence Homology
IF 2.156
引用数 50
WOS 分野 MICROBIOLOGY
リソース情報
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