RRC ID 7144
著者 Hotta Y, Teramoto K, Sato H, Yoshikawa H, Hosoda A, Tamura H.
タイトル Classification of genus Pseudomonas by MALDI-TOF MS based on ribosomal protein coding in S10-spc-alpha operon at strain level.
ジャーナル J Proteome Res
Abstract We have proposed a rapid phylogenetic classification at the strain level by MALDI-TOF MS using ribosomal protein matching profiling. In this study, the S10-spc-alpha operon, encoding half of the ribosomal subunit proteins and highly conserved in eubacterial genomes, was selected for construction of the ribosomal protein database as biomarkers for bacterial identification by MALDI-TOF MS analysis to establish a more reliable phylogenetic classification. Our method revealed that the 14 reliable and reproducible ribosomal subunit proteins with less than m/z 15,000, except for L14, coded in the S10-spc-alpha operon were significantly useful biomarkers for bacterial classification at species and strain levels by MALDI-TOF MS analysis of genus Pseudomonas strains. The obtained phylogenetic tree was consisted with that based on genetic sequence (gyrB). Since S10-spc-alpha operons of genus Pseudomonas strains were sequenced using specific primers designed based on nucleotide sequences of genome-sequenced strains, the ribosomal subunit proteins encoded in S10-spc-alpha operon were suitable biomarkers for construction and correction of the database. MALDI-TOF MS analysis using these 14 selected ribosomal proteins is a rapid, efficient, and versatile bacterial identification method with the validation procedure for the obtained results.
巻・号 9(12)
ページ 6722-8
公開日 2010-12-3
DOI 10.1021/pr100868d
PMID 20945934
MeSH Bacterial Proteins / analysis* Bacterial Proteins / genetics DNA, Bacterial / chemistry DNA, Bacterial / genetics Databases, Protein Molecular Sequence Data Operon Phylogeny Protein Subunits / analysis Protein Subunits / genetics Pseudomonas / classification Pseudomonas / genetics Pseudomonas / metabolism* Ribosomal Proteins / analysis* Ribosomal Proteins / genetics Sequence Analysis, DNA Species Specificity Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
IF 4.074
引用数 29
WOS 分野 BIOCHEMICAL RESEARCH METHODS
リソース情報
一般微生物 JCM 6156 JCM 13061